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目的：研究血管紧张素原基因（ＡＧＴ）第二外显子Ｍ２３５Ｔ等位基因的多态性与高血压之间的关系。方法： 应用多聚酶链反应（ＰＣＲ）结合限制性酶切方法对１０５例健康体检者与１０２例原发性高血压（ＥＨ）患者进行基因突变的检测。结果：（１）ＥＨ患者Ｔ２３５ 等位基因频率（０．４４５）高于对照组（０．３２３），Ｐ＜ ０．０５。在男性ＥＨ患者与男性对照组中差别更为明显（Ｐ＜ ０．０１）；（２）在有家族史的ＥＨ 患者中，Ｍ２３５Ｔ 突变基因型（ＴＴ型）频率高于正常对照组（４２．１％ ＶＳ１８．８％ ，Ｐ＜０．０５）。结论：（１）ＡＧＴ基因的突变与ＥＨ的发病具有相关性。对男性ＥＨ影响可能更大；（２）在有家族史的高血压患者中， ＡＧＴ２３５的ＴＴ基因型与ＥＨ有关
Objective: To investigate the relationship between the polymorphism of M235T allele of the second exon of angiotensinogen gene (AGT) and hypertension. Methods: The gene mutations were detected in 105 healthy subjects and 102 patients with essential hypertension (EH) by polymerase chain reaction (PCR) and restriction enzyme digestion. Results: (1) The frequency of T235 allele in EH patients (0.445) was higher than that in control group (0.323), P <0.05. (P <0.01); (2) The frequency of M235T mutation genotype (TT) in EH patients with family history was higher than that in the control group (42). 1% VS18.8%, P <0.05). Conclusion: (1) The mutation of AGT gene is associated with the pathogenesis of EH. The impact on male EH may be greater; (2) TTT genotype of AGT235 is associated with EH in hypertensive patients with family history