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目的分析儿童外周血白细胞形态参数的变化及其临床意义。方法设定运用Sysmex XE-2100全自动血细胞分析仪(简称XE-2100分析仪)检验血常规的180例门诊表观健康查体儿童(年龄≤15岁)为研究对象,回顾性收集其白细胞形态参数数据。按照年龄0~岁、1~岁、2~岁、6~岁、12~15岁分组,分析研究各白细胞形态参数的变化。结果研究观察各年龄组儿童中性粒细胞散射荧光度即颗粒度(NEUT-X)为1 355.0±41.5、1 348.3±22.5、1 348.4±51.3、1 339.3±51.6、1 335.8±46.2,各组之间差异无统计学意义(P=0.292);中性粒细胞核酸荧光强度即核的大小(NEUT-Y)为495.6±28.2、477.6±38.6、486.5±40.2、481.3±32.2、479.9±39.6,各组之间差异无统计学意义(P=0.412);淋巴细胞核酸荧光强度(LYMPH-Y)为713.6±46.3、708.3±60.3、711.0±61.2、712.8±72.3、711.6±75.3,各组间差异无统计学意义(P=0.115);淋巴细胞散射荧光强度(LYMPH-X)为896.3±36.2、854.6±19.3、858.4±17.6、860.1±15.7、851.3±18.2,各组间差异有统计学意义(P=0.008),其中0~岁组与其他各组两两比较差异有高度统计学意义(P<0.001)。结论小于1岁的健康儿童外周血淋巴细胞颗粒度较其他年龄健康儿童高,具有较高的自身免疫抵抗能力。
Objective To analyze the changes of morphological parameters of peripheral blood leucocytes in children and its clinical significance. Methods The 180 healthy outpatients (≤15 years old) in outpatient routine blood tests were enrolled in this study. The leukocyte morphology was retrospectively collected by Sysmex XE-2100 automatic hematology analyzer (XE-2100 analyzer) Parameter data. According to the age 0 ~ year, 1 ~ year old, 2 ~ year old, 6 ~ year old, 12 ~ 15 year old group, analyze the change of each white blood cell morphological parameter. Results The results showed that the neutrophil scattering fluorescence, particle size (NEUT-X) of children of all age groups was 1 355.0 ± 41.5,1 348.3 ± 22.5,1 348.4 ± 51.3,1 339.3 ± 51.6,1 335.8 ± 46.2, (P = 0.292). Neutrophil fluorescence intensity (NEUT-Y) was 495.6 ± 28.2, 477.6 ± 38.6, 486.5 ± 40.2, 481.3 ± 32.2, 479.9 ± 39.6, There was no significant difference among the groups (P = 0.412). The fluorescence intensity of lymphocytes (LYMPH-Y) was 713.6 ± 46.3,708.3 ± 60.3,711.0 ± 61.2,712.8 ± 72.3,711.6 ± 75.3, (P = 0.115). The fluorescence intensity of lymphocyte scattering (LYMPH-X) was 896.3 ± 36.2, 854.6 ± 19.3, 858.4 ± 17.6, 860.1 ± 15.7 and 851.3 ± 18.2, respectively. There was significant difference among the groups P = 0.008). There was a significant difference between the 0 ~ group and other groups (P <0.001). Conclusion The healthy children younger than 1 year old have higher peripheral blood lymphocyte particle size than healthy children of other ages and have higher autoimmune resistance.