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目的:探讨天麻素(Gastrodin,GAD)对大鼠心脏缺血再灌注损伤的保护作用及其机制。方法:将大鼠随机分为假手术组(Sham组),缺血/再灌注损伤组(MI/R组)和天麻素低、中、高剂量组(GAD组)。通过结扎冠状动脉左前降支构建心肌缺血再灌注损伤模型。测定各组大鼠乳酸脱氢酶(LDH)、肌酸激酶(CK)、天门冬氨酸氨基转移酶(AST)、心肌梗死面积、心脏收缩和舒张功能、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、白细胞介素(IL)-6、IL-1β和肿瘤坏死因子(TNF)-α的表达。结果:天麻素预处理可明显减少心肌梗死面积以及降低LDH、CK、AST、MDA、IL-6、IL-1β和TNF-α表达。此外,天麻素预处理还可改善心脏收缩和舒张功能,增加CAT、GPx和SOD活性。结论:天麻素预处理可通过抑制炎症反应和氧化应激而减轻心脏缺血再灌注损伤。
Objective: To investigate the protective effect of gastrodin (GAD) on myocardial ischemia-reperfusion injury in rats and its mechanism. Methods: The rats were randomly divided into Sham group, MI / R group and gastrodin low, medium and high dose groups (GAD group). Myocardial ischemia-reperfusion injury model was established by ligation of the left anterior descending coronary artery. The levels of lactate dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST), myocardial infarct size, systolic and diastolic function, malondialdehyde (MDA) (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), interleukin (IL) -6, IL-1β and tumor necrosis factor (TNF) -α . Results: Gastrodin preconditioning could significantly reduce the area of myocardial infarction and decrease the expression of LDH, CK, AST, MDA, IL-6, IL-1β and TNF-α. In addition, gastrodin preconditioning can also improve cardiac contractile and diastolic function, increase CAT, GPx and SOD activity. Conclusion: Gastrodin preconditioning can relieve cardiac ischemia-reperfusion injury by inhibiting inflammatory reaction and oxidative stress.