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目的建立亲水作用液相色谱-串联质谱法快速测定食品中的罂粟壳残留生物碱的分析方法。方法样品中的罂粟壳成分采用优化后的Qu ECh ERS方法进行提取,BEH HILIC色谱柱分离(2.1 mm×100 mm,1.7μm),最后采用串联质谱仪对吗啡、可待因、罂粟碱、那可丁及蒂巴因进行检测。结果吗啡、可待因在2.0~100ng/mL范围内线性关系良好,罂粟碱、那可丁、蒂巴因在0.10~5.0 ng/mL范围内线性关系良好。平均加标回收率(n=6)在70%~120%之间,相对标准偏差小于12%。吗啡、可待因、粟碱、那可丁、蒂巴因的检出限分别为10、10、3.8、3.8、7.5μg/kg。结论该方法简便、快速、灵敏,可用于食品中残留罂粟壳成分的检测。
OBJECTIVE To establish a method for the rapid determination of opium poppy residue alkaloids in food by hydrophilic interaction liquid chromatography-tandem mass spectrometry. Methods The poppy shell constituents in the samples were extracted by the optimized Qu ECh ERS method and separated on a BEH HILIC column (2.1 mm × 100 mm, 1.7 μm). Finally, tandem mass spectrometry was used for morphine, codeine, papaverine, Buttin and testin for testing. Results There was a good linear relationship between morphine and codeine in the range of 2.0-100 ng / mL. Papaverine, nocturnine and tobacin showed good linearity in the range of 0.10-5.0 ng / mL. The average spike recoveries (n = 6) ranged from 70% to 120% with relative standard deviations less than 12%. The detection limits of morphine, codeine, base, nocturnine and tetbine were 10, 10, 3.8, 3.8 and 7.5 μg / kg, respectively. Conclusion The method is simple, rapid and sensitive and can be used for the determination of the residual opium poppy in food.