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我们对部分中药的干扰素诱生作用进行了初步实验,现报告如下。一、选用中药:黄芪、香菇各取50g,分别加蒸馏水250ml浸泡二小时,煎15分钟,取汁过滤备用;干晒参30g,加蒸馏水250ml,隔水蒸45分钟,取汁过滤备用;茵栀黄注射液、参三七注射液均为上海中药一厂产品。二、实验材料与方法:1.诱生病毒与攻击病毒。诱生病毒:用新城鸡瘟病毒(NDV-F)系无毒株,(血凝滴度稳定在1:640～1:1280间)。攻击病毒:滤疱性口炎病毒(VSV)。诱生病毒及攻击病毒均取自中国医学科学院病毒所。2.诱生细胞与测定细胞:诱生细胞用人脐带全血中的白细胞,加肝素抗凝;测定细胞用传代人胚肺成纤维细胞或入胚肌皮成纤维细胞。
We conducted preliminary experiments on the induced effects of interferons in some Chinese medicines. The current report is as follows. First, the use of Chinese medicine: Astragalus, mushrooms take 50g, respectively, add distilled water 250ml soak two hours, fry 15 minutes, juice extraction and filtration spare; dry sun Jian 30g, add distilled water 250ml, steam for 45 minutes, juice extraction spare; Shuhuang injection and Shenqi injection are all products of Shanghai No.1 Traditional Chinese Medicine Factory. Second, the experimental materials and methods: 1. induced virus and attack the virus. Induced virus: Newcastle disease virus (NDV-F) is a non-toxic strain (hemagglutination titer is stable between 1:640 and 1:1280). Attacks on the virus: Filtered Sputum Virus (VSV). Both induced and challenge viruses were obtained from the Institute of Viral Diseases of the Chinese Academy of Medical Sciences. 2. Inducing cells and measuring cells: Inducing cells to use white blood cells in human umbilical cord blood, adding heparin to anticoagulation; measuring cells using passaged human embryonic lung fibroblasts or embryogenic myogenic skin fibroblasts.