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为了测定竹红菌乙素(HB)在Triton X-100(TX-100)胶束中的光谱性质及增溶情况,采用紫外-可见分光光度计和荧光分光光度计得到HB在该体系下的吸收光谱和荧光发射光谱,通过HB荧光强度的变化得到增溶情况。在TX-100胶束体系中,竹红菌乙素吸收峰的位置稍有红移且峰型变宽,且荧光发射峰的峰型和峰位均没有大的变化,但最大发射峰强度明显降低。在TX-100胶束的临界胶束浓度(CMC)为58.5μmol·L-1时,HB的最大增溶浓度为50μmol·L-1,得到HB在低浓度TX-100胶束体系中的增溶曲线。该测定方法能够为简单生物模型体系提供一些基础参考数据。
In order to determine the spectroscopic properties and solubilization of HB resin in Triton X-100 (TX-100) micelles, the UV-visible spectrophotometer and fluorescence spectrophotometer were used to obtain HB Absorption spectrum and fluorescence emission spectrum, through the HB fluorescence intensity change solubilization situation. In the TX-100 micellar system, the position of the absorption peak of hypocrellin B slightly red-shifted and the peak broadening, and no significant changes in the peak shape and peak position of the fluorescence emission peak, but the maximum emission peak intensity was significantly reduce. When the critical micelle concentration (CMC) of TX-100 micelles was 58.5μmol·L-1, the maximum solubilization concentration of HB was 50μmol·L-1, which resulted in the increase of HB in low concentration TX-100 micellar system Melting curve. This assay provides some basic reference data for a simple biological model system.