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目的建立天然沉香中白木香酸含量的分析方法,研究白木香酸与浸出物含量(%)之间的相关性。方法按《中国药典》(2010年版)一部进行醇溶性浸出物的含量测定和理化鉴别。高效液相色谱法采用CNW Athena C_(18)-WP柱(250mm×4.6mm,5μm),以乙腈-0.1%(体积分数)磷酸水溶液(40∶60)为流动相,流速1.0ml·min~(-1),检测波长217nm,柱温35℃。结果白木香酸在2.000~80.00μg·ml~(-1)(r=0.9999)范围内线性关系良好,平均回收率为96.1%,RSD为3.0%(n=6)。在理化鉴别和浸出物含量合格的情况下,天然沉香中白木香酸与浸出物含量之间呈正比关系。结论高效液相色谱法可准确测定天然沉香中白木香酸的含量,结合白木香酸与浸出物含量之间的相关性,为天然沉香的质量评价提供依据。
Objective To establish a method for the determination of lemongracin in natural aloes and to study the correlation between lemongrass and leaching content (%). Methods According to the “Chinese Pharmacopoeia” (2010 edition) a determination of alcohol-soluble extract and physical and chemical identification. High performance liquid chromatography with CNW Athena C_ (18) -WP column (250mm × 4.6mm, 5μm) with acetonitrile-0.1% phosphoric acid solution (40:60) as mobile phase and a flow rate of 1.0ml · min ~ (-1), detection wavelength 217nm, column temperature 35 ℃. Results The results showed that there was a good linearity in the range of 2.000 ~ 80.00 μg · ml -1 (r = 0.9999) with the average recovery of 96.1% and RSD 3.0% (n = 6). Physical and chemical identification and extractables in the case of qualified, natural alosmell white acid and extract content was proportional to the relationship between. Conclusion High performance liquid chromatography (HPLC) can accurately determine the content of lemongrala in natural aloes, and provide the basis for the quality evaluation of natural aloes with the correlation between the content of leucopenic acid and leucovorin.