通过对西洋参根外植体愈伤组织诱导和培养,筛选出了质地疏松、生长快的愈伤组织无性系;将该愈伤组织接种在液体培养基中进行振荡培养,经多次传代、筛选获得了分散程度好的高产西洋参悬浮细胞系12-1。在此基础上,建立并优化了西洋参悬浮细胞培养工艺体系:温度(24±1)℃,pH值6.0,生长周期是25d,培养4d后再换液继代一次,培养20d后补料。细胞悬浮培养结束后,细胞的生长量及人参皂甙含量分别达到23.65g/L和677.25mg/L。 Through callus induction and culture of root explants of American ginseng, the clones with loose texture and rapid growth were screened out. The callus was inoculated in liquid medium and cultured by shaking. After multiple passages and screening A highly dispersed American Ginseng suspension cell line 12-1 was obtained. On this basis, the establishment and optimization of the American ginseng suspension cell culture process system: temperature (24 ± 1) ℃, pH value 6.0, the growth cycle is 25d, 4d after the culture liquid replacement once again, 20d after feeding. After cell suspension culture, cell growth and ginsenoside content reached 23.65g / L and 677.25mg / L, respectively.