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小麦光温敏雄性不育系是二系法杂交小麦应用技术体系的核心与基础,其育性严格受光周期和温度控制.了解光温敏雄性不育机理将促进二系法杂交小麦育种技术的应用和发展,而筛选控制不育的关键基因是揭示光温敏雄性不育分子机理的前提.由于小麦基因组信息有限,根据小麦与水稻有较高同源性,尝试利用水稻基因组芯片筛选小麦光温敏雄性不育系BS366冷胁迫响应基因.得到9个差异表达基因,这些基因参与基因表达调控、胁迫应答、信号转导、代谢等重要生命过程,为解析小麦光温敏雄性不育机理提供了有益信息.利用雄蕊cDNA半定量PCR法验证表明,NADH(nicotinamide adenine dinucleotide hydrate)脱氢酶亚基4L、锌指富含DHHC(deaf/hard ofhearing connection)结构、线粒体物质运输蛋白、外被体蛋白COPⅠδ(coat proteinⅠδ)亚基和ABC(ATP-binding cassette)转运蛋白5个基因,在低温和对照温度下表达存在明显差异,可作为育性相关候选基因开展下一步研究.
Wheat photo-thermo-sensitive male sterile line is the core and foundation of the second-line hybrid wheat application technology system, its fertility is strictly controlled by photoperiod and temperature. Understanding the photo-thermo-sensitive male sterility mechanism will promote the hybridization of two-line hybrid wheat breeding technology Application and development, and screening of key genes controlling sterility is the premise of revealing the molecular mechanism of photo-thermo-sensitive male sterility.Because wheat genome has limited information, based on the high homology of wheat and rice, Nine stress-responsive genes were obtained from the male sterile line BS366, and these genes were involved in important life processes such as gene expression regulation, stress response, signal transduction and metabolism, which provided useful information for analyzing the photosynthetic male sterile mechanism of wheat The nucleotide sequence of the dehydrogenase subunit NADH (nicotinamide adenine dinucleotide hydrate) was 4L, and the zinc finger was rich in deaf / hard ofhearing connection (DHHC), mitochondrial transport protein and exosome protein COPⅠδ (coat proteinⅠδ) subunit and ABC (ATP-binding cassette) transporter 5 genes, at low temperature and control temperature There are significant differences in expression, as fertility-related candidate genes to carry out the next step.