作者用乳腺癌原发灶细胞膜作免疫原，制备出抗人乳腺癌单克隆抗体ＡＦ９（ＩｇＧ＿１）。初步实验结果表明其识别的抗原为糖蛋白，同时含有脂类成分。免疫印迹的结果表明，ＡＦ９识别的抗原有４条带，分子量分别为５１、５６、６７、７３ｋｄ。免疫组化结果显示，ＡＦ９对乳腺癌特异性较高（１９／２０例），与直肠癌、卵巢癌、胃癌、肺癌及肝癌等有一定的交叉反应，但不与所检的正常乳腺、甲状腺、胃、直肠、肛门皮肤及其它肿瘤反应。 The authors used the primary breast cancer cell membrane as an immunogen to prepare anti-human breast cancer monoclonal antibody AF9 (IgG_1). Preliminary experimental results show that the antigen identified as glycoprotein, also contains lipid components. The results of Western blotting showed that AF9 recognizes four bands with molecular weights of 51, 56, 67 and 73 kd, respectively. Immunohistochemical results showed that AF9 had high specificity for breast cancer (19/20 cases), had some cross-reactivity with rectal cancer, ovarian cancer, gastric cancer, lung cancer and liver cancer, but not with the normal breast, thyroid , Stomach, rectum, anal skin and other tumor reactions.