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目的:比较研究羌活与宽叶羌活的光合特性,为羌活与宽叶羌活引种驯化及栽培管理技术提供理论依据。方法:利用Li-6400型便携式光合作用测定系统,原位测定自然条件生长的羌活与宽叶羌活孕蕾期叶片的净光合速率对光强、CO2浓度的响应及叶绿素荧光参数。结果:①羌活与宽叶羌活叶片的光饱和点(LSP)分别为1 539,1 464μmol.m-2.s-1,最大净光合速率(Pmax)为22.95,19.65μmol.m-2.s-1,表观量子效率(AQY)为0.050 9,0.047 1,羌活的LSP,AQY和Pmax均显著高于宽叶羌活;光补偿点(LCP)分别为17.92,26.69μmol.m-2.s-1,羌活的LCP显著低于宽叶羌活。②羌活与宽叶羌活的CO2补偿点(CCP)分别为33.41,37.82μmol.mol-1,CO2饱和点(CSP)为988,1 150μmol.mol-1,羌活的CCP,CSP显著低于宽叶羌活;羧化效率(CE)为0.059 1和0.045 9,RuBP最大再生能力(Jmax)为28.18,25.32μmol.m-2.s-1,光呼吸速率(Rd)为1.971,1.736μmol.m-2.s-1,羌活的CE,Jmax,Rd均显著高于宽叶羌活。③羌活与宽叶羌活的PSⅡ潜在活性(Fv/Fm)分别为0.821 3,0.825 7,差异不显著,均没有发生光抑制现象。结论:羌活和宽叶羌活均可能属于C3植物,对光照的适应性均较强。但羌活对弱光的利用能力更强,强光合能力使羌活植株同化物的积累显著高于宽叶羌活。
Objective: To compare and study the photosynthetic characteristics of Alfalfa and Alnus citrix, and to provide theoretical basis for the introduction, domestication, cultivation and management techniques of alfalfa livelihood and broad-leaved alfalfa livelihood. Methods: Using the Li-6400 portable photosynthesis assay system, the photosynthetic rate, light intensity, CO2 concentration, and chlorophyll fluorescence parameters of the photosynthetic rate and the photosynthetic rate were measured in situ during the buds growing in natural conditions. RESULTS: 1 The light saturation points (LSP) of Alfalfa leaf and Alnus nepalensis live leaves were 1 539, 1 464 μmol.m-2.s-1, respectively, and the maximum net photosynthetic rate (Pmax) was 22.95, 19.65 μmol.m-2.s. -1, the apparent quantum efficiency (AQY) was 0.050 9, 0.047 1. The LSP livelihood, AQY and Pmax were all significantly higher than those of wide-leaf defoliation. The light compensation points (LCP) were 17.92 and 26.69μmol.m-2.s, respectively. -1, LCP activity was significantly lower than broadleaf activity. 2 COP compensation points (CCP) were 33.41 and 37.82 μmol.mol-1 for C. lucidum and C. oleifera, and 988,1 150 μmol.mol-1 for CO2 saturation point (CSP). The CCP and CSP were significantly lower than that of broad leaf. Carving activity; carboxylation efficiency (CE) of 0.059 1 and 0.045 9, RuBP maximum regeneration capacity (Jmax) was 28.18, 25.32 μmol.m-2.s-1, and photorespiration rate (Rd) was 1.971, 1.736 μmol.m- 2.s-1, Alfalfa live CE, Jmax, Rd were all significantly higher than that of broad-leaved alfalfa live. 3 The activity of PSII (Fv/Fm) was 0.821 3 and 0.825 7. The difference was not significant and no photoinhibition occurred. Conclusion: Both the alfalfa live and the broad-leaf alfalfa may belong to C3 plants, and their adaptability to light is strong. However, the ability to use weak light was stronger, and the strong photosynthetic capacity enabled the accumulation of assimilate from the alfalfa plants to be significantly higher than that of the broad-leaved alfalfa.