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目的:探讨丹参多酚预处理对大鼠心肌缺血再灌注损伤的保护作用及其可能的机制。方法:成年雄性SD大鼠缺血前1h给予丹参多酚(20mg/kg),结扎冠脉前降支缺血30min后,恢复灌注3h;应用2,3,5-氯化三苯基四氮(TTC)法检测心肌梗死面积;试剂盒检测乳酸脱氢酶(LDH)、肌酸激酶(CK)和髓过氧化物酶(MPO);心肌细胞的凋亡采用原位脱氧糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法检测;Western blot检测高迁移率族蛋B1(HMGB1)表达。结果:与对照组相比,丹参多酚预处理显著减小了心肌梗死面积,降低了心肌细胞凋亡和LDH、CK等血清心肌坏死标记物的表达(P<0.05)。丹参多酚预处理也显著降低了MPO的表达(P<0.05)。同时,丹参多酚预处理抑制了缺血再灌注引起的HMGB1的表达(P<0.05)。结论:丹参多酚预处理可以减轻心肌缺血再灌注损伤并抑制心肌细胞凋亡,其抑制缺血再灌注诱导的HMGB1的表达可能与这种保护作用相关。
Objective: To investigate the protective effect of Salvia miltiorrhiza polyphenols preconditioning on myocardial ischemia-reperfusion injury in rats and its possible mechanism. Methods: Adult male Sprague-Dawley rats were treated with Salviae miltiorrhiza polyphenols (20 mg / kg) 1 h before ischemia and ligation of the anterior descending coronary artery for 30 min followed by 3 h reperfusion. 2,3,5-triphenyltetrazolium chloride (TTC) method was used to detect the area of myocardial infarction. The kit was used to detect lactate dehydrogenase (LDH), creatine kinase (CK) and myeloperoxidase (MPO). Cardiomyocyte apoptosis was detected by in situ deoxyribonucleotidyl transferase Transferase-mediated nick end labeling (TUNEL) assay was used to detect the expression of high mobility group box protein B1 (HMGB1). Results: Compared with the control group, Salvia miltiorrhiza polyphenol pretreatment significantly reduced myocardial infarct size, decreased myocardial cell apoptosis and the expression of LDH, CK and other markers of myocardial necrosis (P <0.05). Salvia miltiorrhiza polyphenol pretreatment also significantly reduced the expression of MPO (P <0.05). At the same time, Salvia miltiorrhiza polyphenol pretreatment inhibited the expression of HMGB1 induced by ischemia-reperfusion (P <0.05). CONCLUSION: Salvia miltiorrhiza polyphenols preconditioning can relieve myocardial ischemia-reperfusion injury and inhibit cardiomyocyte apoptosis, and its inhibitory effect on ischemia-reperfusion-induced HMGB1 expression may be related to this protective effect.