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目的选择测定新生儿高胆红素血症血清胆红素的适宜方法。方法用重氮法和钒酸盐法对30例不同浓度血清总胆红素(TBI)分别进行测定并对结果进行统计学相关和回归分析。结果重氮法和钒酸盐法的结果具有正相关性关系(r=0.9991),然而两种方法所得结果并非对应。回归方程Y=5.7272+0.6791X;表现为随着浓度增高,2种方法所得结果差异越大。结论1.两种方法具有高度相关性。2.对于新生儿高胆红素血症患者的胆红素测定,钒酸盐法的测定结果和临床症状更为吻合。建议临床使用钒酸盐法测定。
Objective To choose a suitable method for the determination of serum bilirubin in neonates with hyperbilirubinemia. Methods 30 cases of different concentrations of serum total bilirubin (TBI) were measured by diazo method and vanadate method, and the results were statistically related and regression analysis. Results There was a positive correlation between the results of diazotization and vanadate (r = 0.9991), however, the results obtained by the two methods were not corresponding. The regression equation Y = 5.7272 + 0.6791X; the higher the concentration, the greater the difference between the two methods. Conclusion 1. The two methods are highly relevant. 2. For neonatal hyperbilirubinemia bilirubin determination, vanadate assay results and clinical symptoms more in line. Recommended clinical use of vanadate assay.