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目的:探讨低氧对喉癌Hep-2细胞增殖及凋亡的影响。方法:模拟人体实体肿瘤低氧环境,应用MTT法检测低氧组与常氧组6、12、24、36h细胞增殖率,流式细胞学方法检测低氧组与常氧组6、12、24、36h细胞凋亡率。结果:常氧与低氧细胞增殖率随时间延长均呈逐渐升高趋势;常氧组与低氧组6、12、24、36h各时间点下比较于24h差异有统计学意义(P<0.01)。低氧组细胞凋亡率于6、12、24h较常氧组低(P<0.01),于36h较常氧组高(P<0.05)。结论:低氧可促进细胞增殖,且细胞增殖率可随时间延长逐渐增高。低氧能够抑制细胞凋亡,但持续缺氧还可以促进细胞凋亡。
Objective: To investigate the effect of hypoxia on the proliferation and apoptosis of laryngeal carcinoma Hep-2 cells. Methods: To simulate the hypoxia environment of human solid tumors, the proliferation rate of 6, 12, 24, 36 h cells in hypoxia and normoxia groups was detected by MTT assay. Flow cytometry was used to detect the proliferation of hypoxic and normoxia groups 6, 12, 24 , 36h apoptosis rate. Results: The proliferation rate of normoxia and hypoxia cells tended to increase with time; the normoxia group and hypoxia group had a significant difference at 6, 12, 24 and 36 hours (P <0.01) ). The apoptosis rate of hypoxia group was lower than that of normoxia group at 6, 12 and 24 hours (P <0.01), and higher than that of normoxia group at 36 hours (P <0.05). Conclusion: Hypoxia can promote cell proliferation, and cell proliferation rate can be increased with time. Hypoxia can inhibit apoptosis, but persistent hypoxia can also promote apoptosis.