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目的探讨奥曲肽对人卵巢癌耐顺铂细胞株SKOV3/DDP裸鼠移植瘤生长的影响。方法将SKOV3/DDP细胞接种雌性BALB/c-nu/nu裸鼠右腋前皮下构建移植瘤动物模型,成瘤后随机分成4组。奥曲肽组(A):奥曲肽100μg.kg-1.d-1,用药28 d;顺铂组(B):顺铂4 mg.kg-1.d-1,成瘤后第1、8、15、22天用药;联合组(C):奥曲肽和顺铂联用;对照组(D):生理盐水对照。用药4周后处死裸鼠,取肿瘤标本计算瘤重和瘤体积,并采用免疫组织化学法检测瘤块中生长抑素受体2(SSTR2)和表皮生长因子受体(EGFR)的表达。结果各用药组肿瘤标本的瘤重、瘤体积均低于D组,C组的瘤重、瘤体积低于A、B组(P<0.01)。各组移植瘤细胞均有SSTR2表达;EGFR表达量D组显著高于其他各组(P<0.01),C组及A组EGFR表达量低于其他两组(P<0.01)。结论奥曲肽能抑制人卵巢癌耐顺铂细胞株SKOV3/DDP的裸鼠移植瘤的生长,并与顺铂有协同作用。
Objective To investigate the effect of octreotide on the growth of transplanted tumor in human ovarian cancer cell line SKOV3 / DDP. METHODS: SKOV3 / DDP cells were inoculated subcutaneously into the right axillary of female BALB / c nu nu mice to construct the xenograft tumor model. Octreotide group (A): Octreotide 100μg.kg-1.d-1, for 28 days; cisplatin group (B): cisplatin 4 mg.kg-1.d-1, , 22 days medication; combination group (C): octreotide combined with cisplatin; control group (D): saline control. The nude mice were sacrificed at 4 weeks after treatment, tumor weight and tumor volume were taken from the tumor samples, and the expression of somatostatin receptor 2 (SSTR2) and epidermal growth factor receptor (EGFR) in the tumor mass was detected by immunohistochemistry. Results The tumor weight and the tumor volume of the tumor specimens in each treatment group were lower than those in the D group. The tumor weight in the C group was lower than that in the A and B groups (P <0.01). The expression of SSTR2 in each group was higher than that in other groups (P <0.01). The expression of EGFR in group C and group A was lower than the other two groups (P <0.01). Conclusion Octreotide can inhibit the growth of transplanted tumor in nude mice bearing cisplatin-resistant cell line SKOV3 / DDP and has a synergistic effect with cisplatin.