论文部分内容阅读
我们应用人结肠癌裸鼠原位移植瘤模型建立CO_2气腹及气腹后早期腹腔化疗进行实验观察,现将结果报道如下。一、材料与方法1．实验动物：BLAB/C nu/nu裸小鼠52只(上海斯莱克实验动物公司提供),雌性,鼠龄5～6周,体重17～19g,在SPF级条件下饲养。2．细胞和主要药物：人结肠癌细胞LoVo(上海细胞生物研究所提供),体外培养于DMEM完全培养液(含10%小牛血清及100U/ml青、链霉素)。ZT胶,5-氟尿嘧啶(5-Fu),亚叶酸钙(CF)分别购自广州白云医用胶公司、南通精华制药公司、北京双鹭药业公司。
We use human orthotopic transplantation tumor model in nude mice to establish CO_2 pneumoperitoneum and pneumoperitoneum after early intraperitoneal chemotherapy experimental observations, the results reported below. First, materials and methods 1. Experimental animals: 52 BLAB / C nu / nu nude mice (provided by Slack Laboratory Animal Company, Shanghai), female, aged 5-6 weeks, weighing 17-19g, were housed under SPF conditions. 2. Cells and the main drug: human colon cancer cells LoVo (Shanghai Institute of Cell Biology), cultured in DMEM complete medium (containing 10% fetal bovine serum and 100U / ml green, streptomycin). ZT glue, 5-fluorouracil (5-Fu) and leucovorin (CF) were purchased from Guangzhou Baiyun Medical Adhesive Co., Ltd., Nantong Essence Pharmaceutical Co., Ltd. and Beijing Shuanglu Pharmaceutical Co., Ltd. respectively.