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采用凝胶过滤方法对番茄叶霉病菌6个生理小种所产生的毒素进行了分离、纯化,并利用叶片针刺法分别对其蛋白组分进行了生物活性测定,以探讨番茄叶霉病菌不同生理小种毒素组分对番茄叶片的致病力的影响。结果表明,每个生理小种毒素的纯化峰蛋白组分中只有一个蛋白组分能使番茄叶片褪绿,分别为来自生理小种1.2.3的第4个峰蛋白组分(记作:1.2.3(4),下同)、1.2.4(2)、1.2.3.4(2)、1.3(2)、1.3.4(2)和1.4(2)。说明这几个峰蛋白组分对番茄叶片有一定的致病力,而其它峰蛋白组分对番茄叶片则没有明显致病力。透射电子显微镜观察结果显示,叶绿体膨胀或变形、基质片层部分消解,其中1.2.3.4(2)处理的叶绿体解体最彻底。
The toxins produced by 6 physiological races of Fusarium solani were isolated and purified by gel filtration. Bioassay was carried out on their protein components by leaf-acupuncture to explore the effects of different pathogen of Fusarium graminearum Influences of Physiocarcinogen Components on Pathogenicity of Tomato Leaves. The results showed that only one protein component of the purified peak protein fraction of each of the physiocarotoxins chlorosis the tomato leaf, which is respectively the fourth peak protein component from 1.2.2 .3 (4), same below), 1.2.4 (2), 1.2.3.4 (2), 1.3 (2), 1.3.4 (2) and 1.4 (2). These peak protein components of tomato leaves have a certain degree of virulence, while other peak protein components of tomato leaves have no significant pathogenicity. Transmission electron microscopy showed that the chloroplast was swollen or deformed, and the matrix lamella was partially digested. The chloroplast disintegrated in 1.2.3.4 (2) was the most complete.