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目的 探讨微小RNA (miR)-10b对肝癌细胞侵袭转移能力的影响.方法 通过Transwell小室基质侵袭和迁移实验检测不同肝癌细胞系的侵袭和迁移能力.qRT-PCR方法检测各肝癌细胞系中miR-10b的表达.通过体外转染法将化学合成的miR-10b的模拟体(mimics)瞬时转染至低表达miR-10b的HepG2细胞并检测侵袭和迁移能力的变化;瞬时转染miR-10b抑制剂(antagomir)至高表达miR-10b的MHCC97H细胞并检测侵袭和迁移能力的变化.利用免疫荧光染色和电镜观察细胞骨架变化.结果 miR-10b表达水平与肝癌细胞的迁移、侵袭能力相关.迁移、侵袭能力较弱的HepG2细胞miR-10b表达较低,而迁移、侵袭能力较强的MHCC97H细胞miR-10b表达较高.上调HepG2细胞miR-10b后迁移和侵袭能力明显增强,而下调MHCC97H细胞miR-10b后迁移和侵袭能力明显削弱.免疫荧光染色和电镜观察显示,转染miR-10b模拟物后,HepG2细胞的丝状伪足和板状伪足明显增多,而下调MHCC97H细胞miR-10b后,丝状伪足和板状伪足明显减少.结论 miR-10b可通过调控细胞微丝骨架参与人肝癌细胞体外侵袭转移,抑制其表达可能为肝癌治疗提供新的靶点.“,”Objective To investigate the effect of miR-10b on migration and invasion of human hepatocellular carcinoma (HCC) cell lines.Methods Transwell assay was used to evaluate the motility and invasiveness in different HCC cell lines,qRT-PCR was then used to detect the expression levels of miR-10b in different HCC cell lines.Artificial mimics of miR-10b were transiently transfected into HepG2 cells,which have low expression of miR-10b,and then the changes in migration and invasion were evaluated by Transwell assay.Antagomirs of miR-10b (miR-10b-AS) were transiently transfected into MHCC97H cells,which have high expression of miR-10b,and then the changes in migration and invasion were evaluated as well.Cell morphology changes were detected by immunofluorescence and electron microscopy,Results There was a significant correlation of miR-10b expression level with cell motility and invasiveness.Low-level expression of miR-10b was observed in HepG2 cells,which exhibited weak motility and invasiveness; whereas high-level expression of miR-10b was observed in MHCC97H cells,which exhibited strong motility and invasiveness.Up-regulation of miR-10b expression in HepG2 cells increased cell motility and invasiveness,whereas inhibition of miR-10b reduced cell motility and invasiveness in MHCC97H cells.Immunofluorescence and electron microscopy results showed that up-regulation of miR-10b in HepG2 cells significantly increased proliferation of filopodia and lamellipodia,whereas inhibition of miR-10b decreased filopodia and lamellipodia amount in MHCC97H cells.Conclusion miR-10b is involved in the invasion and metastasis of HCC cell through regulation of cytoskeleton in vitro and inhibition of miR-10b is likely to be a new molecular target to block metastasis.