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为探讨甲硫氨基酸对亚热带红壤硝化作用和N2O排放的影响,选择福建省建瓯市万木林保护区的山地红壤为研究对象,在土壤饱和持水量(WHC)60%和90%的条件下,开展室内培养试验.试验分为对照(CK)、添加甲硫氨基酸(M)、甲硫氨基酸和硫酸铵(MA)、甲硫氨基酸和亚硝酸钠(MN)、甲硫氨基酸和葡萄糖(MC)5个处理.结果表明:与对照相比,M处理使土壤NH4+-N平均含量显著提高0.8%~61.3%,而NO3--N含量显著降低13.2%~40.7%;60%WHC条件下,MC处理土壤NO2--N含量高于M处理,MA、MN处理NO3--N含量高于M处理,且MN处理高于MA处理,M处理于试验后期最低,表明甲硫氨基酸抑制了硝化作用的亚硝化过程.碳添加处理使甲硫氨基酸在一定程度上降低NH4+-N含量,抑制了土壤自养硝化,并且甲硫氨基酸和碳源共同作用下NO3--N含量变化与土壤水分条件有关,在90%WHC条件下,碳加入后反硝化作用更明显;而NO3--N含量降低不足以表明是异养硝化受到抑制所致.甲硫氨基酸在一定程度上促进土壤N2O的释放,90%WHC条件下较60%WHC条件下释放量更大,且葡萄糖添加的促进效果更明显.
In order to investigate the effect of methionine on nitrification and N2O emission in subtropical red soil, the red soil of mountainous area in Wanmulin Nature Reserve, Jian’ou City, Fujian Province, was selected as the research object. Under conditions of 60% and 90% of soil saturated water content (WHC) (M), methionine and ammonium sulfate (MA), methionine and sodium nitrite (MN), methionine and glucose (MC) 5 treatments.The results showed that compared with the control, M treatment significantly increased soil NH4 + -N content by 0.8% -61.3%, while NO3 - N content decreased significantly by 13.2% -40.7% The content of NO2 - N in soil was higher than that of M, the content of NO3 - N in MA and MN was higher than that of M, and the content of MN was higher than that of MA. The content of NO2 - N was the lowest in late stage of experiment. Nitrosation.Carbon addition could reduce the content of NH4 + -N and inhibit the autotrophic nitrification of the soil, and the change of NO3 - N content was related to the soil moisture condition under the combined action of methionine and carbon source, Under the conditions of 90% WHC, the denitrification was more obvious after the addition of carbon, while the content of NO3 - N decreased less It showed that heterotrophic nitrification is inhibited due. Methylthio amino acids promote the release of N2O to some extent, a greater release at 60% WHC conditions under condition than 90% WHC, and the effect of promoting glucose added is more obvious.