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目的观察杂环胺代表物2-氨基-1-甲基-6-苯基-咪唑并[4,5-b]吡啶(PhIP)对雄性Wistar大鼠不同组织蛋白质氧化损伤。方法将20只健康成年清洁级Wistar雄性大鼠随机分为4组,分别为对照(pH=4.5的55%乙醇-生理盐水)组和低(5 mg/kg)、中(10 mg/kg)、高(15 mg/kg)剂量PhIP染毒组,每组5只。按5 ml/kg进行一次性灌胃染毒。染毒后24 h,测定心、肺、肝、肾组织蛋白质羰基(PCO)、DNA和蛋白质交联(DPC)率。结果与对照组比较,各剂量PhIP染毒组大鼠心组织和仅高剂量PhIP染毒组大鼠肺、肝、肾组织中PCO含量升高(P<0.01,P<0.05),各剂量PhIP染毒组大鼠肺组织和中、高剂量PhIP染毒组大鼠肝组织以及高剂量PhIP染毒组大鼠心、肾组织中DPC率升高(P<0.05,P<0.01)。且PhIP染毒剂量与大鼠心、肺、肝、肾组织中的PCO含量和DPC率均呈正相关(r≥0.94,P<0.05)。PCO含量增加的次序为心>肺>肝和肾。结论 PhIP可引起大鼠不同组织蛋白质的氧化损伤,这种效应存在组织差异性。
OBJECTIVE To observe the oxidative damage of protein in different tissues of male Wistar rats with 2-amino-1-methyl-6-phenyl-imidazo [4,5-b] pyridine (PhIP) Methods Twenty healthy adult Wistar male rats were randomly divided into 4 groups: control (55% ethanol - saline at pH 4.5) and low (5 mg / kg) and medium (10 mg / kg) , High (15 mg / kg) dose PhIP exposure group, 5 rats in each group. Press 5 ml / kg for one-time gavage. Twenty-four hours after exposure, protein carbonyl (PCO), DNA and protein cross-link (DPC) rates in heart, lung, liver and kidney were measured. Results Compared with the control group, the contents of PCO in lung, liver and kidney of rats in PhIP group were higher than those in control group (P <0.01, P <0.05) The DPC rate in the lung tissue and the medium and high dose PhIP groups and in the high and low dose PhIP groups were increased (P <0.05, P <0.01). PhIP exposure dose was positively correlated with PCO content and DPC rate in heart, lung, liver and kidney (r> 0.94, P <0.05). The order of increase in PCO content was heart> lung> liver and kidney. Conclusion PhIP can cause oxidative damage to proteins in different tissues of rats. There is tissue difference in this effect.