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目的:探讨附子理中汤干预脾肾阳虚型溃疡性结肠炎(Ulcerative colitis,UC)可能的药效环节。方法:SPF级大鼠48只按随机数字表法分为正常对照组、模型组、柳氮磺胺吡啶组、附子理中汤高、中、低剂量组6组。中西药联合制备符合脾肾阳虚证的免疫化学性复合模型,药物灌肠干预后用ELISA法检测IL-6、IL-8在血清中的含量,取结肠组织,免疫组化染色(SP)法检测ICAM-1的表达,并观察其病理组织学情况。结果:中药各剂量组与模型组比较,血清中IL-6、IL-8水平、结肠黏膜病理评分及其ICAM-1表达均有所下降,各项检测指标表明附子理中汤高剂量组的疗效优于柳氮磺胺吡啶组,差异有统计学意义。结论:附子理中汤对脾肾阳虚型UC疗效确切,作用机制可能与降低血清促炎症因子及细胞间黏附因子的表达有关。
Objective: To investigate the possible pharmacological actions of Fuzi Lizhong Tang on ulcerative colitis (UC). Methods: Forty-eight SPF rats were randomly divided into six groups: normal control group, model group, sulfasalazine group and Fuzi Lizhong Tang high, medium and low dose groups. Western medicine combined with preparation of immunochemical chemical compound model of spleen and kidney yang deficiency, the content of IL-6, IL-8 in serum after the intervention of drug enema was measured by ELISA, and the colon tissue and immunohistochemistry (SP) The expression of ICAM-1 was detected and the histopathology was observed. Results: Compared with the model group, the levels of IL-6, IL-8 in serum and the pathological score of colonic mucosa and the expression of ICAM-1 in the Chinese herbal medicine groups decreased. The detection indexes showed that the high dose of Fuzi Lizhong Decoction The curative effect is superior to the sulfasalazine group, the difference was statistically significant. Conclusion: Fuzi Lizhong Decoction is effective in treating spleen-kidney-yang-deficiency type UC and its mechanism may be related to the decrease of serum proinflammatory cytokines and the expression of intercellular adhesion molecule.