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疣粒野生稻(O. meyeriana (Zoll. etMor. exSteud.))对水稻白叶枯病具有高度的抗病性, 为转移这种抗病性, 进行了栽培稻+疣粒野生稻不对称体细胞杂交. 以软X射线处理过的疣粒野生稻原生质体作供体, 来源于栽培稻品种02428的原生质体经碘乙酰胺(IOA)失活后作受体, 采用PEG法诱导二者的原生质体融合. 由于代谢功能互补, 融合物经培养后得到623块愈伤组织, 最终分化出72株再生植株. 这些融合植株形态上与栽培稻接近, 采用微卫星分子标记进行了杂种鉴定. 细胞学分析表明, 融合杂种根尖细胞染色体数目在27~38条之间变化. 在成株期对融合植株进行了白叶枯病接种鉴定, 结果显示疣粒野生稻对水稻白叶枯病的抗性成功地转移到栽培稻中.
O. meyeriana (Zoll et et al. Ex Steud.) Has a high resistance to bacterial leaf blight in rice, and in order to transfer this disease resistance, Cell hybridization.With the soft X-ray treated wild rice protoplast as donor, the protoplasts from Oryza sativa cultivar 02428 were inactivated by iodoacetamide (IOA) Protoplast fusion.Due to the complementary metabolic function, 623 callus tissues were obtained after the fusion, and 72 regenerated plants were finally differentiated.The hybrid plants were morphologically similar to cultivated rice, and the hybrids were identified by microsatellite markers. The results showed that the number of chromosomes in the root tip cells of the hybrids changed from 27 to 38. The inoculation of the blight on the fused plants at the adult stage showed that the resistance of the warty wild rice to bacterial leaf blight Sex is successfully transferred to cultivated rice.