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通过离子交换-抑制电导法,监测螺旋藻培养过程主要营养盐的消耗,同时考察了生物量累积与营养盐消耗的关系。实验结果表明,螺旋藻培养过程中营养源消耗量为:N源(NO-3)>P源(HPO2-4)>S源(SO2-4),基本不消耗Cl-。培养过程中的营养盐消耗符合Doseresp模型,实际值与模型拟合相关系数R2达到0.98以上。螺旋藻生物量干质量对NaNO3(YX/N)、K2HPO4(YX/P)、K2SO4(YX/S)的得率系数分别为1.86、21.30、37.02g/g。合理监测螺旋藻培养过程中的营养盐消耗,有利于控制藻的生长和质量,为补料策略的制定提供理论依据。
The ion exchange - inhibition conductance method was used to monitor the nutrient consumption of Spirulina platensis during cultivation. At the same time, the relationship between biomass accumulation and nutrient consumption was examined. The experimental results showed that the nutrient consumption of Spirulina cultivation was: NO-3> HPO2-4> SO2-4, and no consumption of Cl-. The nutrient consumption during cultivation accorded with Doseresp model, and the correlation coefficient R2 between the actual value and the model reached above 0.98. The yield coefficients of NaNO3 (YX / N), K2HPO4 (YX / P) and K2SO4 (YX / S) were 1.86, 21.30 and 37.02 g / g, respectively. Reasonable monitoring of spirulina nutrient consumption during cultivation is conducive to controlling the growth and quality of algae, providing a theoretical basis for the formulation of feeding strategy.