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目的研究敌敌畏(O,O-dimethyl-O-2,2-dichlorovinylphosphate,DDVP)对人神经母细胞瘤细胞(SK-N-SH)的氧化损伤作用。方法 MEM培养基培养SK-N-SH细胞,细胞计数测定SK-N-SH细胞群体倍增时间;不同浓度(0~120μmol/L)DDVP作用SK细胞48 h后,MTT法测定SK-N-SH细胞48 h半数抑制浓度(IC50);巴氏染色后观察细胞形态变化;同时分别检测细胞内超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量的变化。结果SK-N-SH细胞群体倍增时间(36.28±1.46)h;48 h半数抑制浓度(IC50)为(105.22±5.80)μmol/L;随着DDVP染毒浓度的增高,细胞内MDA的含量逐渐增高,SOD、CAT活性逐渐降低,呈现明显的剂量—效应关系(P<0.05)。结论 DDVP对SK-N-SH细胞有明显的氧化损伤作用。
Objective To investigate the oxidative damage of human neuroblastoma cells (SK-N-SH) by O-dimethyl-O-2,2-dichlorovinylphosphate (DDVP) METHODS: SK-N-SH cells were cultured in MEM medium and the doubling time of SK-N-SH cells was determined by cell counting. After SK-N-SH cells were treated with DDVP at different concentrations (0 ~ 120μmol / (IC50). The cell morphology was observed after pasteurization. At the same time, the activities of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) The change. Results The population doubling time of SK-N-SH cells was (36.28 ± 1.46) h, and the IC50 of 48 h was (105.22 ± 5.80) μmol / L. With the increase of DDVP concentration, (P <0.05). The activity of SOD and CAT decreased gradually with dose-effect relationship (P <0.05). Conclusion DDVP has obvious oxidative damage on SK-N-SH cells.