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目的检测基质金属蛋白酶(MMPs)MMP-2、MMP-9在先天性二叶型主动脉瓣中的表达,探讨MMP-2、MMP-9在先天性二叶型主动脉瓣病变中的作用。方法选取因先天性二叶型主动脉瓣伴主动脉瓣狭窄(AS)和(或)主动脉瓣关闭不全(AI)行主动脉瓣置换术切取的儿童二叶型主动脉瓣瓣膜为研究组(n=12,男11例,女1例,年龄10～18岁,平均16.7岁,单纯AS3例、单纯AI8例、AS-AI1例),病例来源为2003-01—2005-12华中科技大学同济医学院同济医院住院患儿;以同期性别、年龄相近,无心血管系统及胶原系统疾病、意外死亡患儿的正常三叶型主动脉瓣(n=8,男6例,女2例,年龄在1～17岁之间,平均年龄9.1岁)为对照组,采用HE染色和免疫组织化学方法,观察先天性二叶型主动脉瓣组织学变化,对MMP-2、MMP-9蛋白质表达行半定量分析。结果正常主动脉瓣可见MMP-2、MMP-9低水平表达,MMPs在先天性二叶型主动脉瓣组、对照组表达的平均灰度值:MMP-2分别为(68.31±6.91)、(107.31±23.39),(P<0.05);MMP-9分别为(64.47±3.98)、(116.28±6.99),(P<0.05);先天性二叶型主动脉瓣组MMP-2、MMP-9表达较正常对照组显著性增强。结论先天性二叶型主动脉瓣中MMP-2、MMP-9表达水平增高,MMP-2、MMP-9可能参与先天性二叶型主动脉瓣细胞外基质(ECM)降解、重构,导致主动脉瓣结构和功能障碍,在先天性二叶型主动脉瓣病变的发生发展中起重要作用。
Objective To investigate the expression of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in congenital two-lobe aortic valve and to explore the role of MMP-2 and MMP-9 in congenital two-lobed aortic valve disease. Methods Aortic valve replacement in children with congenital bileaflet aortic valve stenosis (AS) and / or aortic regurgitation (AI) was selected as the study group (n = 12, 11 males and 1 females, aged 10 to 18 years, mean 16.7 years old, simple AS3 cases, simple AI8 cases, AS-AI1 cases), the source of the cases was 2003-01-2005-12 Huazhong University of Science and Technology Tongji Medical College Tongji Hospital inpatients; normal tri-type aortic valve (n = 8, 6 males and 2 females, age At the age of 1 to 17 years old with an average age of 9.1 years) as the control group. HE staining and immunohistochemistry were used to observe the histological changes of congenital bileaflet aortic valve. The expressions of MMP-2 and MMP-9 protein Semi-quantitative analysis. Results The expression of MMP-2 and MMP-9 was low in normal aortic valve. The average gray value of MMPs in congenital bileaflet aortic valve group was (68.31 ± 6.91), ( 107.31 ± 23.39, P <0.05). The MMP-9 levels in the congenital two-leaf aortic valve group were (64.47 ± 3.98) and (116.28 ± 6.99) Compared with the normal control group, the expression was significantly enhanced. Conclusions The expression of MMP-2 and MMP-9 in congenital biaxial aortic valve is increased. MMP-2 and MMP-9 may be involved in the degradation and remodeling of congenital bile duct aortic valve extracellular matrix (ECM) Aortic valve structure and dysfunction in congenital aortic valve disease plays an important role in the occurrence and development.