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首先采用无皂乳液聚合法获得了含有羧基官能团的聚苯乙烯-甲基丙烯酸共聚物(poly(styrene-comethacrylic acid),PSMAI),然后利用静电纺丝技术制备了PSMAI纳米纤维.并以酰胺结合的方式,在PSMAI纤维表面固载辣根过氧化物酶(HRP).采用SEM、FTIR等手段表征了PSMAI纤维的表面微观形貌和化学特性.研究了HRP在纤维表面的固载时间与酶活性的定量关系,对比分析了酶固载前后的热稳定性,存储稳定性和PH稳定性.并以邻甲氧基苯酚作为水体污染的模型物,考察了固载HRP对邻甲氧基苯酚的去除效率.结果表明固载后的HRP相比游离HRP环境适应性能有较大提升.固载化HRP对邻甲氧基苯酚(OMP)催化反应120 min去除效率达80.2%,明显高于自由HRP去除效率(50%),有望应用于酚类水体污染的治理.
Firstly, soap-free emulsion polymerization was used to obtain poly (styrene-co-comethacrylic acid) (PSMAI) with carboxyl functional groups, and then PSMAI nanofibers were prepared by electrospinning. (HRP) was immobilized on the surface of PSMAI fiber.The surface morphology and chemical properties of PSMAI fiber were characterized by SEM and FTIR.The immobilization time of HRP on the surface of fiber was compared with that of HRP Activity, the thermal stability, storage stability and PH stability before and after enzyme immobilization were compared and analyzed.With o-methoxyphenol as a model of water pollution, the effects of HRP loaded with o-methoxyphenol .The results showed that the immobilized HRP had a better ability to adapt to free HRP than the free HRP.The immobilized HRP had an efficiency of 80.2% at 120 min for the reaction of o-methoxyphenol (OMP), which was significantly higher than that of free HRP removal efficiency (50%), is expected to be applied to the treatment of phenolic water pollution.