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目的观察人参二醇组皂苷(panoxadiol saponin PDS)体外对DU145前列腺癌细胞增殖及凋亡的影响。方法采用MTT比色法检测不同浓度PDS对DU145细胞增殖活力的影响。吖啶橙染色观察PDS诱导细胞凋亡情况;流式细胞仪分析细胞周期及凋亡,并计算细胞平均凋亡率;细胞免疫化学和Western blot技术观察细胞内激活的caspase3的表达。结果经中(100mg/L)、高(200mg/L)剂量的PDS处理后,DU145细胞生长受到不同程度抑制,且呈现剂量依赖性;吖啶橙荧光染色可见经中、高剂量PDS处理后的细胞呈明显凋亡形态,并随药物剂量增加,凋亡细胞数增多;流式细胞术结果显示,PDS可明显提高细胞的凋亡率,呈剂量依赖关系;免疫细胞化学染色和Western blot结果,随着PDS剂量增加,细胞内激活的caspase3的表达上调。结论 PDS体外对DU145细胞增殖具有明显的抑制作用,其机制可能与诱导细胞凋亡有关。
Objective To observe the effects of panaxadiol saponin PDS on the proliferation and apoptosis of DU145 prostate cancer cells in vitro. Methods MTT colorimetric assay was used to detect the effect of different concentrations of PDS on the proliferation of DU145 cells. Acridine orange staining was used to observe the cell apoptosis induced by PDS. Cell cycle and apoptosis were analyzed by flow cytometry, and the average apoptosis rate was calculated. The expression of caspase 3 was observed by immunocytochemistry and Western blot. Results After PDS treatment of 100 mg / L and 200 mg / L, the growth of DU145 cells was inhibited in a dose-dependent manner. Fluorescence staining of acridine orange showed that the growth of DU145 cells was inhibited by medium- and high-dose PDS The cells showed obvious apoptotic morphology, and the number of apoptotic cells increased with the increase of the drug dose. The results of flow cytometry showed that PDS could significantly increase the apoptosis rate in a dose-dependent manner. Immunocytochemical staining and Western blot results showed that, With the increase of PDS dose, the expression of intracellular activated caspase3 was up-regulated. Conclusion PDS can significantly inhibit the proliferation of DU145 cells in vitro, which may be related to the induction of apoptosis.