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目的:观察桃红四物丸对兔皮片移植模型成活及组织VEGFR-2含量的影响,探讨其对皮片移植成活的作用机制。方法:将40只清洁级新西兰大白兔随机分为2组,均制成兔皮片移植模型,造模后治疗组予桃红四物丸灌胃治疗,对照组予等容积蒸馏水灌胃,每天2次,连续14d。通过组织病理学检查、血管密度检测和免疫组化染色的方法,观察比较2组术后11d和14d移植皮片组织病理学评分、血管密度及VEGFR-2含量。结果:治疗组术后11d和14d的组织病理学评分、血管密度和VEGFR-2含量均高于对照组,差异均有统计学意义(P<0.05)。结论:桃红四物丸可通过改善组织病理学情况、促进血管新生、增加VEGFR-2含量,从而促进移植皮片成活。
Objective: To observe the effect of Taohongsiwu pill on survival and tissue VEGFR-2 content in rabbit skin graft model and to explore its mechanism of action on skin graft survival. Methods: Forty clean-grade New Zealand white rabbits were randomly divided into two groups. Rabbit skin graft models were made. The model group was treated with Taohongsiwu pill by intragastric administration. The control group was given an equal volume of distilled water, Times, for 14 days. Histopathological examination, vascular density test and immunohistochemical staining were used to observe the histopathological score, vascular density and VEGFR-2 content in the skin grafts on the 11th and 14th postoperative day. Results: Histopathological score, vascular density and VEGFR-2 level in the treatment group were significantly higher than those in the control group on the 11th and 14th day after operation (P <0.05). Conclusion: Taohong Siwu pill can improve graft survival by improving histopathology, promoting angiogenesis, increasing VEGFR-2 content.