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目的:观察雌激素缺乏骨丢过程中T细胞亚群(Th1/Th2/Th17/Treg)变化,探讨其在雌激素缺乏骨丢失中的作用,为临床治疗提供新的靶点与途径。方法:30只BALB/c小鼠随机分为正常组、假手术组及卵巢切除组,各10只。ELISA法检测血清雌二醇(E2)水平,双能X线骨密度仪检测股骨骨密度(BMD),流式细胞仪胞内外双染色法检测脾脏单个核细胞Th1/Th2/Th17/Treg亚群比例。结果:与正常小鼠及假手术小鼠比较,卵巢切除小鼠E2、BMD显著降低(P<0.05),Th1、Th17亚群比例明显升高(P<0.05),Th2、Treg亚群比例明显降低(P<0.05),Th1/Th2比值及Th17/Treg升高(P<0.05);BMD与E2水平、Th2、Treg亚群比例呈显著正相关(P<0.05),与Th1、Th17亚群比例呈显著负相关(P<0.05)。结论:T细胞亚群偏移参与了雌激素缺乏导致的骨丢失,逆转T细胞亚群失衡可能成为防治雌激素缺乏骨丢失的有效靶点与途径。
Objective: To observe the changes of T cell subsets (Th1 / Th2 / Th17 / Treg) in the process of estrogen deficiency bone loss and to explore its role in estrogen deficiency bone loss, and to provide new targets and approaches for clinical treatment. Methods: Thirty BALB / c mice were randomly divided into normal group, sham operation group and ovariectomized group, 10 in each. Serum estradiol (E2) level was detected by ELISA, bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry and the Th1 / Th2 / Th17 / Treg subsets of spleen mononuclear cells were detected by flow cytometry proportion. Results: The levels of E2 and BMD in ovariectomized mice were significantly decreased (P <0.05), the ratios of Th1 and Th17 subpopulations were significantly increased (P <0.05), and the proportion of Th2 and Treg subpopulations was significantly higher in ovariectomized mice (P <0.05), Th1 / Th2 ratio and Th17 / Treg increased (P <0.05). There was a significant positive correlation between BMD and E2, Th2 and Treg subsets (P <0.05) The proportion was significantly negatively correlated (P <0.05). CONCLUSION: T-cell subsets are involved in bone loss induced by estrogen deficiency. Reversing the imbalance of T-cell subsets may be an effective target and approach for prevention and treatment of bone loss caused by estrogen deficiency.