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研究干扰素γ对人肺腺癌细胞系A5 49的毒性作用及它们与化疗药物联用应用的协同效应 ,以期为肺癌的临床治疗提供新的方向。方法 :用MTT显色法检测不同浓度组药物对A5 49细胞的毒性作用 ,并计算其抑制率。结果 :干扰素γ能直接抑制人肺腺癌A5 49细胞的生长 ,最大抑制率分别为 40 47%。低剂量的干扰素γ(10 0U ml、5 0U ml)与化疗药物联用后的抑制率高于单用化疗药物 ,其中以与MMC、VCR、5 FU联用后的效果较好。结论 :干扰素γ对A5 49细胞具有直接抑制效应 ,但敏感性不高。干扰素γ与部分化疗药物联合应用对A5 49细胞系具有显著的协同抗肿瘤效应。这些可能为肺癌的临床治疗提供实验依据
To study the toxic effects of interferon gamma on human lung adenocarcinoma cell line A5 49 and their synergistic effect with chemotherapeutic drugs, in order to provide a new direction for the clinical treatment of lung cancer. METHODS: The cytotoxic effects of different concentration groups on A5 49 cells were detected by MTT colorimetry, and the inhibition rate was calculated. RESULTS : Interferon gamma can directly inhibit the growth of human lung adenocarcinoma A5 49 cells, with a maximum inhibition rate of 40 47%. Low-dose interferon gamma (100 U ml, 50 U ml) was more effective than chemotherapeutic agents alone when combined with chemotherapeutic agents, and combined with MMC, VCR, and 5 FU had better results. Conclusion : Interferon gamma has a direct inhibitory effect on A5 49 cells, but its sensitivity is not high. The combination of interferon gamma and some chemotherapeutics has a significant synergistic anti-tumor effect on the A5 49 cell line. These may provide experimental basis for the clinical treatment of lung cancer