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利用超高效液相色谱与LTQ-Orbitrap-MS串联质谱仪的尿液代谢组学方法,研究黄连、胆黄连对热证模型大鼠药效作用机制的差异性,探讨胆黄连炮制的科学性。采用大鼠灌胃附子、干姜、肉桂水煎液15 d并皮下注射干酵母混悬液致热证模型,给药15 d后采集各组大鼠造模后0~6,6~12,12~24 h的尿样;采用主成分分析(PCA)、偏最小二乘-判别分析(PLSDA)法等技术进行数据处理。正常组与模型组在0~6,6~12 h达到分离,12~24 h出现重叠,分离趋势不明显;黄连组和胆黄连组0~6 h大鼠尿样与模型组分离,接近于正常组;黄连组和胆黄连组在0~6,6~12 h大鼠尿样有分离趋势。鉴定30个与热证相关的差异代谢物,结果表明,黄连经猪胆汁炮制后对热证模型大鼠的整体药效作用发生改变,胆黄连解热作用具有多靶点、起效快、作用强度较强的特点,主要通过对胆碱能神经递质、氨基酸代谢、嘌呤代谢的调节发挥解热作用。
Urine metabolomics method of ultra-high performance liquid chromatography coupled with LTQ-Orbitrap-MS tandem mass spectrometry was used to study the difference of the mechanism of action of Coptis Chinensis and Radix. The rats were fed with aconite, ginger and cinnamon decoction for 15 days and injected subcutaneously with dry yeast suspension to induce the heat syndrome model. After 15 days of administration, rats in each group were harvested for 0-6, 6-12, The urine samples were collected for 12-24 h. The data were processed by principal component analysis (PCA), partial least squares-discriminant analysis (PLSDA) and other techniques. The normal group and the model group were separated at 0 ~ 6, 6 ~ 12 h, overlapped at 12 ~ 24 h, and the separation tendency was not obvious. The urine samples of rats in Huanglian group and Huanghuang group for 0 ~ 6 h were separated from the model group In the normal group, the urine samples of Coptis chinensis group and Cholelithromycin group showed a trend of separation from 0 to 6 and 6 to 12 h. Identification of 30 heat syndrome-related differences in metabolites, the results show that Coptis chinensis biloba prepared after the heat-induced model of the overall effect of pharmacological changes in rats, anti-pyretic effect of bile-diphtheria has multiple targets, rapid onset, the role Strong intensity characteristics, mainly through the cholinergic neurotransmitter, amino acid metabolism, purine metabolism play a role in antipyretic.