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药物敏感试验方法 1961年世界卫生组织专家委员会调查了世界各国结核菌药物敏感试验方法,人体有以下三种: 1.绝对浓度法:在含药培养基中各接种10~(-1)、10~(-2)、10~(-3)、10~(-1)、10~(-5)mg/ml的结核菌液0.02ml(一白金环),在37℃暖箱内培养4～6周,记录在不含药对照培养基内生长20～100个结核菌落的接种菌量下的各种药物的最低抑菌浓度。或简化为只接种一种固定的接种菌量(10~(-2)、10~(-3)或10~(-1)mg),记其各药的最低抑制浓度。这种方法精确性差,只能得到供临床参考的大致结果。我国目前多应用此法的简化法,方法
Drug sensitivity test methods 1961 WHO Expert Committee investigated the world’s drug susceptibility testing methods for TB, the body has the following three: 1. Absolute concentration method: in the drug-containing medium of each vaccination 10 ~ (-1), 10 0.02 ml (a Platinum ring) of the tubercle bacilli in 10-4, 10-3, 10-1, and 10-5 mg / ml in a 37 ° C. incubator, After 6 weeks, the minimum inhibitory concentration (MIC) of each drug was recorded at the inoculation amount of 20 to 100 tuberculosis colonies grown without drug control medium. Or simplified to inoculate only one kind of fixed inoculum (10-2, 10-3, or 10-1 mg), and record the minimum inhibitory concentration of each drug. The accuracy of this method is poor and can only give general results for clinical reference. At present, China simplifies the method and method of applying this method