论文部分内容阅读
目的了解广西艾滋病病毒感染/艾滋病(HIV/AIDS)患者隐孢子虫感染情况及虫株类型。方法收集广西南宁、桂林、钦州、百色及河池等地285名HIV/AIDS患者和南宁150名HIV阴性人群粪便样本,分别采用改良抗酸染色法和基于18S rRNA基因巢式PCR方法进行隐孢子虫检测,并对巢氏PCR产物进行测序和同源性比对,以明确基因型。结果广西HIV/AIDS患者隐孢子虫感染率为0.70%(2/285),其中伴有慢性腹泻者感染率为6.67%(2/30),HIV阴性者隐孢子虫感染率为0(0/150),后两者差异有统计学意义(P=0.002)。2例合并感染隐孢子虫的HIV/AIDS患者均来自桂林,虫株基因鉴定为安氏隐孢子虫和人隐孢子虫。结论广西HIV/AIDS患者存在隐孢子虫合并感染,感染虫株为安氏隐孢子虫和人隐孢子虫。
Objective To understand the status of Cryptosporidium infection and strain types in Guangxi HIV / AIDS patients. Methods A total of 285 HIV / AIDS patients in Nanning, Guilin, Qinzhou, Baise, Hechi, Guangxi and 150 HIV-negative females from Nanning were collected. The faecal samples were collected by modified acid-fast staining and nested PCR based on 18S rRNA gene. Detection, and the nest of PCR products were sequenced and homologous comparison to clear the genotype. Results The prevalence of Cryptosporidium in HIV / AIDS patients in Guangxi was 0.70% (2/285), of which 6.67% (2/30) were associated with chronic diarrhea and 0 (0 / 150), the difference between the two was statistically significant (P = 0.002). Two cases of HIV / AIDS patients infected with Cryptosporidium were from Guilin, and the strains were identified as Cryptosporidium parvum and Cryptosporidium parvum. Conclusions There is a co-infection of Cryptosporidium in HIV / AIDS patients in Guangxi. The infected strains are Cryptosporidium parvum and Cryptosporidium parvum.