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目的观察α干扰素(IFNα)治疗慢性乙型肝炎(CHB)过程中外周血树突状细胞(DCs)亚群的变化及其与临床疗效的关系。方法慢性乙型肝炎患者25例,应用α干扰素治疗24周,随访24周。利用流式细胞技术对患者外周血mDC和pDC的百分比和绝对数进行随访观察。结果治疗有效组13例,无效组12例。IFNα治疗CHB患者24周时HBVDNA阴转率、HBeAg阴转率、抗HBe阳转率分别为35%、40%、10%;48周时分别为40%、33.3%、20%。外周血mDC和pDC的百分比和绝对数在IFNα治疗后均呈下降趋势。其中,治疗有效组患者在治疗前,治疗后12周、24周、48周时mDC和pDC绝对数分别为(16.5±5.51)×106/L、(9.86±5.2)×106/L、(9.20±3.19)×106/L、(10.0±3.64)×106/L和(5.91±2.35)×106/L、(4.25±2.00)×106/L、(3.30±1.55)×106/L、(4.32±1.59)×106/L,分别进行多组间方差分析,均有统计学意义;无效组患者在治疗前,治疗后12周、24周、48周时mDC和pDC绝对数分别为(14.4±6.62)×106/L、(14.0±5.27)×106/L、(10.40±4.6)×106/L、(12.3±5.23)×106/L和(5.10±1.72)×106/L、(4.06±1.67)×106/L、(3.89±1.25)×106/L、(4.06±8.12)×106/L,经统计学处理,均无统计学意义。结论FNα治疗可以获得持续性病毒学及生化学应答。IFNα治疗可导致体内?
Objective To observe the changes of subsets of peripheral blood dendritic cells (DCs) during the treatment of chronic hepatitis B (CHB) with IFNα and its relationship with clinical outcome. Methods Twenty-five patients with chronic hepatitis B were treated with IFN-α for 24 weeks and followed up for 24 weeks. The percentage and absolute number of mDC and pDC in peripheral blood of patients were observed by flow cytometry. Results The effective treatment group of 13 cases, ineffective group of 12 cases. The HBVDNA negative rate, HBeAg negative rate and anti-HBe positive rate were 35%, 40% and 10% respectively at 24 weeks after treatment with IFNα, and 40%, 33.3% and 20% respectively at 48 weeks. The percentage and absolute number of mDC and pDC in peripheral blood showed a decreasing trend after IFNα treatment. Among them, the absolute numbers of mDC and pDC before treatment, before treatment, 12 weeks, 24 weeks and 48 weeks after treatment were (16.5 ± 5.51) × 106 / L, (9.86 ± 5.2) × 106 / L and ± 3.19 × 106 / L, (10.0 ± 3.64) × 106 / L and (5.91 ± 2.35) × 106 / L, (4.25 ± 2.00) × 106 / L, (3.30 ± 1.55) × 106 / L, ± 1.59) × 106 / L respectively, all of which were statistically significant. The absolute numbers of mDC and pDC before treatment, before treatment, at 12 weeks, 24 weeks and 48 weeks after treatment were (14.4 ± 6.62 × 106 / L, 14.0 ± 5.27 × 106 / L, 10.40 ± 4.6 × 106 / L, 12.3 ± 5.23 × 106 / L and 5.10 ± 1.72 × 106 / 1.67) × 106 / L, (3.89 ± 1.25) × 106 / L, (4.06 ± 8.12) × 106 / L, respectively, which were not statistically significant after statistical analysis. Conclusion FNα therapy can achieve sustained virological and biochemical responses. IFNα treatment can lead to in vivo?