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目的:通过观察枸杞多糖对去卵巢大鼠肝脏中HMGCR、L-FABP及LPL蛋白表达的影响,探究枸杞多糖对去卵巢大鼠脂代谢的作用机制。方法:30只SPF级健康雌性SD大鼠随机分为假手术组、去卵巢组、补佳乐组、枸杞多糖高剂量组及枸杞多糖低剂量组。全自动生化分析仪检测大鼠血脂水平,HE染色观测各组大鼠肝脏组织形态学变化,Western Blot方法检测肝脏中HMGCR、L-FABP及LPL蛋白表达。结果:血脂检测发现,与假手术组相比,去卵巢组大鼠血清TC、LDL-C水平显著升高、HDL-C水平显著下降(P<0.01);与去卵巢组相比,补佳乐组与枸杞多糖高剂量组TC水平显著下降(P<0.01),补佳乐组与枸杞多糖高、低剂量组LDL-C水平显著下降(P<0.01 or P<0.05)。HE染色发现,去卵巢组可见散在的脂肪空泡,高剂量枸杞多糖治疗后脂肪空泡减少。Western Blot结果显示,与假手术组相比,去卵巢组大鼠肝脏HMGCR、L-FABP蛋白表达上调,LPL蛋白表达下调(P<0.01);与去卵巢组相比,枸杞多糖高剂量组HMGCR、L-FABP与LPL蛋白表达呈相反趋势(P<0.01)。结论:高剂量枸杞多糖可能通过下调肝脏中HMGCR与L-FABP,上调LPL蛋白表达改善脂代谢紊乱。
OBJECTIVE: To investigate the effect of LBP on the expression of HMGCR, L-FABP and LPL protein in the livers of ovariectomized rats and to explore the mechanism of LBP on lipid metabolism in ovariectomized rats. Methods: Thirty SPF healthy female SD rats were randomly divided into sham-operated group, ovariectomized group, high-performance group, LBP high-dose group and LBP low-dose group. The blood lipid level of rats was detected by automatic biochemical analyzer. The morphological changes of liver tissue were observed by HE staining. The expressions of HMGCR, L-FABP and LPL protein in liver were detected by Western Blot. Results: Compared with sham operation group, serum TC and LDL-C levels in ovariectomized group were significantly increased and HDL-C level was significantly decreased (P <0.01). Compared with ovariectomized group, The levels of TC in the high-dose group and the high-dose LB group were significantly decreased (P <0.01). The LDL-C levels in the high and low dose LB group and LB group were significantly decreased (P <0.01 or P <0.05). Hematoxylin and eosin (HE staining) showed that there were scattered fat vacuoles in the ovariectomized group and reduced fat vacuoles after treatment with high-dose LBP. The results of Western Blot showed that compared with the sham operation group, the expression of HMGCR and L-FABP in the livers of ovariectomized rats was increased and the expression of LPL protein was decreased (P <0.01). Compared with ovariectomized rats, HMGCR , L-FABP and LPL protein expression showed the opposite trend (P <0.01). CONCLUSION: High-dose LBP may improve lipid metabolism disorder by down-regulating HMGCR and L-FABP in liver and up-regulating LPL protein expression.