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目的:探索咳嗽变异性哮喘(CVA)痰瘀互结病证结合大鼠动物模型的构建方法。方法:模拟中医病因,以幼龄大鼠肺脾气虚,烟熏致痰浊内生,长期激怒联合高脂饮食致气滞血瘀,建立痰瘀互结中医证候模型;模拟西医病因病理,卵蛋白氢氧化铝致敏,辣椒素激发,建立西医大鼠CVA模型。观察大鼠一般状态、自发活动情况、咳嗽反应、肺支气管病理组织形态、支气管肺泡灌洗液(BALF)嗜酸性粒细胞计数。结果:痰瘀互结模型组大鼠体质量增长缓慢(P<0.01),自发活动减少(P<0.01),咳嗽次数增加(P<0.01);支气管管腔狭窄,黏膜增厚,肺泡腔增大,黏液分泌增多,大量嗜酸性粒细胞浸润(P<0.01);与模型组比较,痰瘀同治组各项观测指标均有明显改善(P<0.01)。结论:模拟传统中医病因、西医病因病理建立CVA动物模型;以整体观念、微观验证综合评价中医CVA动物模型;以方测证,痰瘀同治反证CVA动物模型。多因素复合作用可成功复制CVA大鼠病证结合动物模型。
Objective: To explore the construction of a rat model of cough variant asthma (CVA) with phlegm and blood stasis syndrome. Methods: To simulate the etiology of traditional Chinese medicine (TCM), the TCM syndromes of phlegm, blood stasis and blood stasis were established by inducing phlegm and blood stasis in young rats, Alveolar aluminum hydroxide sensitization, capsaicin excitation, the establishment of western rat CVA model. The general state of rats, spontaneous activity, cough response, bronchial pathology and bronchoalveolar lavage fluid (BALF) eosinophil count were observed. Results: The body mass of rats in phlegm-blood stasis model group increased slowly (P <0.01), spontaneous activity decreased (P <0.01), cough frequency increased (P <0.01), bronchial stenosis, mucosa thickening, Large, mucus secretion increased, a large number of eosinophil infiltration (P <0.01); Compared with the model group, the observation of phlegm and blood stasis Tongzhi group were significantly improved (P <0.01). Conclusion: The CVA animal model was established by simulating the etiology of traditional Chinese medicine and the etiology and pathogenesis of Western medicine. The CVA animal model was comprehensively evaluated with the concept of holistic concept and microscopic validation. The animal model of CVA was reversed with syndrome differentiation and phlegm-blood stasis syndrome. Multi-factor combination can successfully copy the CVA rat disease syndromes combined with animal models.