异丙酚和氯胺酮对大鼠离体缺血再灌注损伤心肌脂质过氧化的影响

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目的:比较异丙酚和氯胺酮对大鼠离体缺血再灌注损伤心肌脂质过氧化的影响。方法:成年Wistar大鼠18只,雌雄不拘,体重240-300 g,随机分为3组(n=6):心肌缺血再灌注损伤组(I/R组),异丙酚组(P组),氯胺酮组(K组)。采用Langendorff逆灌装置建立离体心脏缺血再灌注模型,将心脏连接至Langendorff逆灌装置,3组均以K-H液平衡灌注10 min后,再分别以K-H液、含30μmol·L-1异丙酚的K-H液、含10μmol·L-1氯胺酮的K-H液灌注10 min,然后全心停灌25 min,再分别以停灌前相同的灌注液恢复灌注30 min。留取冠脉流出液测定总LDH活性;灌注末取左室心肌组织置于2.5%的戊二醛固定,观察心肌的超微结构;心尖部心肌组织留待检测8-异前列腺素和SOD活性。结果:与I/R组比较,P组8-异前列腺素含量降低,SOD活性升高,LDH活性降低(P<0.05);K组8-异前列腺素含量,SOD及LDH活性均无统计学意义(P>0.05);与P组比较,K组8-异前列腺素含量升高,SOD及LDH活性降低(P<0.05);P组心肌超微结构损伤较IR组和K组也明显改善。结论:异丙酚可显著减轻心肌缺血再灌注损伤大鼠的脂质过氧化和心肌缺血再灌注损伤,而氯胺酮没有抗心肌缺血再灌注损伤心肌脂质过氧化的作用。 Objective: To compare the effects of propofol and ketamine on myocardial lipid peroxidation in isolated rat hearts with ischemia-reperfusion injury. Methods: Eighteen adult Wistar rats were randomly divided into three groups (I / R group), propofol group (P group) ), Ketamine group (K group). Langendorff reverse irrigation device was used to establish the model of ischemia-reperfusion in vitro. The heart was connected to Langendorff reverse-irrigation device. All the three groups were infused with KH solution for 10 min. Then KH solution, 30μmol·L-1 isopropyl Phenol KH solution, KH solution containing 10μmol·L-1 ketamine for 10 min, and then stop the whole heart for 25 min, then reperfusion perfusion with the same perfusion fluid before stopping perfusion for 30 min. The total LDH activity was measured by taking coronary effluent. The left ventricular tissue was fixed with glutaraldehyde (2.5%) at the end of perfusion. The ultrastructure of myocardium was observed. The apical myocardial tissue was taken for the detection of 8-isoprostane and SOD activity. Results: Compared with the I / R group, the content of 8-isoprostaglandin, the activity of SOD and the activity of LDH of P group decreased (P <0.05). There was no statistical difference in 8-isoprostane content, SOD and LDH activity in K group (P <0.05) .Compared with P group, the content of 8-isoprostaglandin in K group increased and the activity of SOD and LDH decreased (P <0.05); the myocardial ultrastructure damage of P group also improved obviously compared with IR group and K group . CONCLUSION: Propofol can significantly reduce lipid peroxidation and myocardial ischemia-reperfusion injury in rats with myocardial ischemia-reperfusion injury, while ketamine has no effect on myocardial lipid peroxidation induced by myocardial ischemia-reperfusion injury.
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