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提取鳜鱼病毒核酸 ,分别用DNase、RNase和绿豆芽核酸酶处理表明 ,该病毒为双链DNA病毒 ,用带EcoRⅠ酶切位点的随机引物扩增病毒核酸 ,获得扩增核酸带谱 ,经低熔点琼脂糖回收PCR产物 ,与质粒pUC19连接 ,获得三个重组子 ,已经对两个小插入片断进行了序列分析 ,插入序列分别为 36 9bp和 45 0bp。GenBank检测显示 ,尚未有类似的序列报导。
The DNA extracted from the anchovy virus was treated with DNase, RNase and Mung bean bud nuclease, respectively. The results showed that the virus was a double-stranded DNA virus. The viral nucleic acid was amplified by random primers with EcoR I restriction sites, The low melting point agarose was used to recover the PCR product and ligated with plasmid pUC19 to obtain three recombinants. Two small insertions were sequenced and the insert sequences were 369 bp and 45 bp, respectively. GenBank tests show that there is no similar sequence reported.