在双波长分光光度法中,用计算机选择波长对进行测定,可以获得较好的精确度,这方面已进行了不少工作。作者引入“权系数法”提出一种计算机选择测定波长对的新程序,其中考虑到了仪器测定偏差对测定结果的影响,以波长对的权系数为判据,可选出较为可靠的测定波长对。原理对于双波长分光光度法来说,仪器测定 In dual-wavelength spectrophotometry, computer-selected wavelength pairs are measured for better accuracy, and much work has been done in this area. The author introduced the “weight coefficient method” proposed a computer program to determine the wavelength of the new program, taking into account the instrument measurement bias on the determination of the results, the wavelength of the weight coefficient as a criterion, you can choose a more reliable wavelength pair . Principle For dual-wavelength spectrophotometry, the instrument determination