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目的 研究霍乱毒素 (CTx)对视网膜cAMP水平及视网膜节细胞存活及凋亡的影响。 方法 视神经远端 (颅内 )切断 ,玻璃体内注射CTx,采用荧光逆行示踪技术及原位末端标记 (TUNEL)技术显示存活的视网膜节细胞及节细胞层凋亡的细胞 ,Brown放射免疫法测定各时间点视网膜cAMP的水平。 结果 远端切断视神经后各时间点cAMP水平均低于玻璃体内注射霍乱毒素组。视神经远端切断后视网膜节细胞平均密度明显下降 ,玻璃体内注射霍乱毒素后 ,节细胞平均密度明显提高 ,细胞凋亡数也明显少于单纯视神经切断组。 结论 表明霍乱毒素可提高成年金黄地鼠视网膜cAMP水平并具有促受损视网膜节细胞存活及抗凋亡作用。
Objective To study the effect of CTx on retinal cAMP level and retinal ganglion cell survival and apoptosis. Methods The distal (intracranial) optic nerve was cut off and intravitreal injection of CTx was performed. Fluorescence retrograde tracing technique and TUNEL technique were used to detect the apoptotic cells in the retinal ganglion cells and ganglion cells. Brown radioimmunoassay Retinal cAMP levels at various time points. Results The level of cAMP in the distal optic nerve was significantly lower than that in the vitreous injected cholera toxin group. The average density of retinal ganglion cells decreased significantly after the distal optic nerve was cut off. After the intravitreal injection of cholera toxin, the average cell density was significantly increased, and the number of apoptotic cells was also significantly less than that of the optic nerve alone group. The results showed that cholera toxin can increase retinal cAMP level in adult golden hamster and promote the survival and anti-apoptotic effect of damaged retinal ganglion cells.