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采用滴涂法和循环伏安法制备石墨烯-聚唑类化合物复合膜修饰玻碳电极,分别用循环伏安法和差分脉冲伏安法研究黄嘌呤和鸟嘌呤混合物在修饰电极上的电化学行为。在0.2 mol/L的磷酸盐缓冲液(p H 5.5)中,黄嘌呤和鸟嘌呤混合物在修饰的玻碳电极上具有较好的电化学行为。鸟嘌呤和黄嘌呤分别在4.0×10~(-4)~4.0×10~(-6)mol/L和4.0×10~(-5)~4.0×10~(-8)mol/L范围内有较好的电化学响应,鸟嘌呤和黄嘌呤检出限分别为4.0×10~(-7)mol/L和4.0×10~(-9)mol/L。方法用于同时测定人体尿液中鸟嘌呤和黄嘌呤,回收率分别为102.1%~105.9%和96.9%~106.5%。
The graphene-polyazole composite films modified glassy carbon electrodes were prepared by a drop-coating method and a cyclic voltammetry. The electrochemical behaviors of xanthine and guanine mixtures on the modified electrodes were investigated by cyclic voltammetry and differential pulse voltammetry, respectively behavior. In 0.2 mol / L phosphate buffer (p H 5.5), the mixture of xanthine and guanine has good electrochemical behavior at the modified glassy carbon electrode. The concentrations of guanine and xanthine in the range of 4.0 × 10 -4 to 4.0 × 10 -6 mol / L and 4.0 × 10 -5 to 4.0 × 10 -8 mol / L, respectively The results showed that the detection limits of guanine and xanthine were 4.0 × 10 -7 mol / L and 4.0 × 10 -9 mol / L, respectively. The method was applied to the simultaneous determination of guanine and xanthine in human urine with recoveries of 102.1% -105.9% and 96.9% -106.5%, respectively.