淋巴细胞经培养后微核的检测,作为辐射损伤短期监测染色体损伤的又1种细胞遗传学方法已有不少研究。80年代中期Fenech和Morley,首次使用细胞松驰素B(Cytochalasin B,Cyt-B)建立了人淋巴细胞胞浆分裂阻滞微核测试法(Cytokinesis-block Micronucleus Method),简称CB微核法。此法克服了常规培养微核法的不足,可获得大量易于识别的第1次分裂后的双核细胞,并记录其中的微核。我们应用胞浆分裂阻滞微核测试法,对~(60)Coy-线剂量率致微核效应进行了探讨。实验选用同一健康成年男 Micronucleus detection of lymphocytes cultured as a short-term monitoring of chromosomal damage by radiation damage and a cytogenetic method has been a lot of research. In the mid-1980s, Fenech and Morley first used Cytokinesis-block Micronucleus Test (Cytokinesis-block Micronucleus Method), referred to as CB micronucleus test, for the first time using Cytochalasin B (Cyt-B). This method overcomes the shortcomings of conventional culture micronucleus method, can obtain a large number of easily identifiable first division of the binuclear cells, and record the micronuclei. We used micronucleus test of cytokinesis to investigate the micronucleus effect of ~ (60) Coy-line dose rate. Experimental selection of the same healthy adult male