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目的：研究Ｅｂｓｅｌｅｎ对Ｃｕ２＋、Ｆｅ２＋及巨噬细胞氧化修饰人低密度脂蛋白（ＬＤＬ）的抑制作用及其可能机制。方法：采用紫外分光光度法检测人ＬＤＬ中硫代巴比妥酸反应物（ＴＢＡＲＳ）及共轭双烯（ＣＤ）的含量来表示ＬＤＬ的被氧化程度。结果：Ｃｕ２＋、Ｆｅ２＋及体外培养的小鼠腹腔巨噬细胞（ＭＰＭ）都能氧化修饰人ＬＤＬ，使其中的ＴＢＡＲＳ和ＣＤ含量显著升高。Ｅｂｓｅｌｅｎ（０．５～４．０μｍｏｌ／Ｌ）能剂量依赖性降低人ＬＤＬ中ＴＢＡＲＳ和ＣＤ含量，其中２μｍｏｌ／Ｌ的Ｅｂｓｅｌｅｎ能完全抑制Ｃｕ２＋、Ｆｅ２＋及ＭＰＭ升高ＬＤＬ中ＴＢＡＲＳ含量的作用。单用谷胱甘肽（ＧＳＨ，０．５ｍｍｏｌ／Ｌ）对ＬＤＬ中ＴＢＡＲＳ含量没有显著的影响，但ＧＳＨ能显著增强Ｅｂｓｅｌｅｎ对ＴＢＡＲＳ生成的抑制作用。结论：Ｅｂｓｅｌｅｎ能抑制Ｃｕ２＋、Ｆｅ２＋及ＭＰＭ氧化修饰人ＬＤＬ，ＧＳＨ能明显增强Ｅｂｓｅｌｅｎ的这种抑制作用。
Objective: To study the inhibitory effect of Ebselen on oxidized low density lipoprotein (LDL) in Cu2 +, Fe2 + and macrophages and its possible mechanism. Methods: UV-Vis spectrophotometry was used to determine the degree of oxidation of LDL by detecting the content of thiobarbituric acid reactants (TBARS) and conjugated dienes (CDs) in human LDL. Results: Both Cu2 +, Fe2 + and mouse peritoneal macrophages (MPM) cultured in vitro could oxidatively modify human LDL and the contents of TBARS and CD in them were significantly increased. Ebselen (0.5 ~ 4.0μmol / L) could reduce TBARS and CD in human LDL in a dose-dependent manner. Ebselen at 2μmol / L could completely inhibit the effect of Cu2 +, Fe2 + and MPM on TBARS in LDL. Glutathione alone (GSH, 0.5 mmol / L) had no significant effect on the TBARS content in LDL, but GSH significantly enhanced the inhibitory effect of Ebselen on TBARS production. Conclusion: Ebselen can inhibit the oxidative modification of human LDL by Cu2 +, Fe2 + and MPM. GSH can significantly enhance this inhibitory effect of Ebselen.