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透明质酸(HYA)是一种广泛分布在机体内、尤其存在疏松结缔组织中的高分子量多糖。为了了解中耳及内耳组织中透明质酸的分布,选用10只成年SD大鼠,通过过量的戊巴比妥钠腹腔注射将其处死,然后断头,取出颞骨,并打开听泡,在微波固定及微波脱钙后,将软化的颞骨标本制成厚5μm的连续石蜡切片,用高亲和力的生物素标记的HYA结合蛋白作为探针,通过免疫组化方法来研究中耳及内耳HYA的分布。结果发现:在中耳,HYA主要分布在鼓膜松弛部、听小骨、圆窗膜和中耳肌肉的肌内膜中,咽鼓管鼓口上皮下亦有
Hyaluronic acid (HYA) is a high molecular weight polysaccharide widely distributed in the body, especially loose connective tissue. In order to understand the distribution of hyaluronic acid in the middle ear and inner ear tissues, 10 adult SD rats were selected and killed by intraperitoneal injection of excess pentobarbital sodium, then decapitated, the temporal bone was removed, After fixed and microwave decalcification, the softened temporal bone specimens were made into serial 5-μm-thick paraffin sections, and the high-affinity biotin-labeled HYA-binding protein was used as a probe to investigate the distribution of middle ear and inner ear HYA by immunohistochemistry . The results showed that: in the middle ear, HYA mainly in the tympanic membrane relaxation, ossicles, round window membrane and middle ear muscle in the endometrium, eustachian tube also have subepithelial