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目的探讨子宫内膜异位症（ＥｎｄｏｍｅｔｒｉｏｓｉｓＥＭＳ）的免疫状态与中医辨证分型的关系。方法采用间接免疫荧光法检测ＥＭＳ患者外周血中的Ｔ细胞亚群、Ｂ、ＮＫ、ＩＬ－２Ｒ＋细胞，并用酶联免疫双抗体夹心法检测外周血及腹腔液中的ＴＮＦ－α，并与中医辨证分型进行相关关系分析。结果以气滞血瘀、寒凝血瘀、湿热瘀阻三组组成的实证组ＣＤ＋４／ＣＤ＋８值升高，而肾虚血瘀组除此变化外，Ｂ、ＩＬ－２Ｒ＋细胞百分率增高，外周血及腹腔液ＴＮＦ－α浓度也显著增高。结论ＣＤ＋４／ＣＤ＋８值与多因素逐步回归分析后发现，ＥＭＳ患者外周血中ＣＤ＋４／ＣＤ＋８值与中医辨证分型密切相关。
Objective To investigate the relationship between the immune status of endometriosis (EMs) and syndrome differentiation of traditional Chinese medicine (TCM). Methods The T lymphocyte subsets, B, NK and IL-2R + cells in peripheral blood of patients with EMS were detected by indirect immunofluorescence. TNF-α in peripheral blood and peritoneal fluid was detected by enzyme-linked immunosorbent assay (ELISA) Dialectical classification of the correlation analysis. Results The positive rate of CD + 4 / CD + 8 increased in the positive group of three groups, including qi stagnation and blood stasis, cold coagulation and blood stasis, and damp-heat stasis. Except for the changes of kidney deficiency and blood stasis group, the percentage of B and IL- Liquid TNF-α concentration was also significantly higher. Conclusion CD + 4 / CD + 8 and multi-factor stepwise regression analysis showed that CD + 4 / CD + 8 in peripheral blood of EMS patients is closely related to TCM syndrome differentiation.