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目的:探讨黄皮叶提取物对哮喘大鼠炎症反应中Th1/Th2细胞因子平衡的调节作用。方法:将健康雄性SD大鼠48只,随机分为6组,分别为正常组、哮喘模型组、地塞米松组(1.5 mg·kg-1·d-1)、黄皮叶提取物低、中、高剂量组(520,1 040,2 080 mg·kg-1·d-1)。除正常组外,其余各组分别在第1,7天采用卵清蛋白致敏法ih致敏液,并于第15天开始,各给药组大鼠每天在灌胃给药后1 h进行雾化激发,连续1周;正常组及模型组以生理盐水灌胃代替。分别采用ELISA法测定大鼠血清中一氧化氮(NO),白三烯D4(LTD4),白细胞介素-4(IL-4),γ-干扰素(IFN-γ)的含量、硝酸还原法测肺组织NO含量;RT-PCT法测定肺组织中IL-4,IFN-γmRNA表达及观察肺组织病理学改变。结果:与正常组比较,模型组大鼠血清中NO,IL-4,LTD4的含量明显升高,IFN-γ的含量降低,肺组织NO含量明显升高,大鼠肺组织中IL-4 mRNA表达明显升高,IFN-γmRNA表达明显降低(P<0.01);与模型组比较,黄皮叶提取物低、中、高剂量组均能明显减少血清中NO,IL-4的含量,升高IFN-γ的含量(P<0.05,P<0.01),黄皮叶提取物高、中剂量组能减少哮喘大鼠肺组织中NO的含量,黄皮叶提取物高剂量组能明显减少哮喘大鼠血清中LTD4的含量(P<0.05,P<0.01),黄皮叶提取物低、中、高剂量组能明显降低哮喘大鼠肺组织中IL-4mRNA表达,增加IFN-γmRNA表达(P<0.05,P<0.01)。结论:黄皮叶提取物能有效的减少哮喘大鼠的炎症反应,其机制可能是通过调节Th1/Th2细胞因子的平衡,从而减轻炎症细胞浸润有关。
Objective: To investigate the regulation of Th1 / Th2 cytokine balance in the inflammation of asthmatic rats induced by Huangpiye extract. Methods: Forty-eight healthy male Sprague-Dawley rats were randomly divided into 6 groups: normal group, asthma model group, dexamethasone group (1.5 mg · kg-1 · d-1) Medium and high dose group (520,1 040 and 2 080 mg · kg-1 · d-1). In addition to the normal group, the remaining groups were treated with ovalbumin sensitized ih sensitized solution on day 1,7, and began on the 15th day, the rats in each treatment group were treated 1 h after gavage Nebulization, continuous 1 week; normal group and model group replaced by saline gavage. The content of nitric oxide (NO), leukotriene D4 (LTD4), interleukin-4 (IL-4) and interferon-γ (IFN- The content of NO in lung tissue was measured. The expression of IL-4 and IFN-γmRNA in lung tissue was measured by RT-PCT and the pathological changes of lung were observed. Results: Compared with normal group, the levels of NO, IL-4 and LTD4 in serum of model group were significantly increased, the content of IFN-γ was decreased and the content of NO in lung tissue was significantly increased. The level of IL-4 mRNA (P <0.01). Compared with the model group, the contents of NO and IL-4 in serum of the low, medium and high dose groups of Huangpiye extract significantly decreased, while the expression of IFN-γmRNA decreased (P <0.05, P <0.01). High and middle dose of Huangpiye extract could reduce the content of NO in the lung tissue of asthmatic rats, and the high dose of Huangpiye extract could significantly reduce the asthma (P <0.05, P <0.01). The contents of IL-4 mRNA and IL-4 mRNA in the lung tissue of asthmatic rats were significantly decreased by low, medium and high dose of Huangpiye extract (P < 0.05, P <0.01). Conclusion: Huangpiye extract can effectively reduce the inflammatory response in asthmatic rats. The mechanism may be related to the regulation of Th1 / Th2 cytokine balance and the reduction of inflammatory cell infiltration.