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采用盆栽试验研究不同浓度(0.5%和1.0%)NaCl胁迫下接种丛枝菌根真菌Glomus mosseae-2对番茄吸水特性的影响,并用realtime-PCR技术分析了根系中5个水孔蛋白基因(LePIP1,LePIP2,LeTRAMP,LeAQP2,LeTIP)的表达。结果表明:盐胁迫下接种丛枝菌根真菌能促进植株生长和对水分的吸收,显著提高叶片相对含水量、叶片水势及根系水导;丛枝菌根真菌和盐胁迫共同调控了5个水孔蛋白基因的表达;与未接菌株相比,接种植株LeAQP2基因表达上调,其余4个基因表达下调。LeAQP2基因在接菌株根中的过量表达与盐胁迫下丛枝菌根真菌提高番茄根系水导有关。
Pot experiments were conducted to investigate the effects of arbuscular mycorrhizal inoculation with Glomus mosseae-2 on the water-absorbing characteristics of tomato under different NaCl stress (0.5% and 1.0% NaCl stress). Five waterhole protein genes (LePIP1 , LePIP2, LeTRAMP, LeAQP2, LeTIP). The results showed that inoculation of arbuscular mycorrhizal fungi under salt stress could promote plant growth and water absorption, and significantly increase relative leaf water content, leaf water potential and root conductance; arbuscular mycorrhizal fungi and salt stress jointly regulated 5 water Porin gene expression; compared with unacceptable strains, inoculated plants LeAQP2 gene expression was up regulated, and the remaining four genes were down-regulated. The overexpression of LeAQP2 gene in the root of the inoculated strain is related to the improvement of root canal conductivity of tomato by arbuscular mycorrhizal fungi under salt stress.