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本试验应用蛋白质双向电泳及质谱技术,对单作和与玫瑰间作的K326叶片蛋白质进行分离鉴定。利用Plant-m PLoc、Gene Ontolog、KOBAS、STRING对所鉴定蛋白进行生物信息学分析。在每份样品分离到的400左右个蛋白质中,MALDI-TOF/TOF MS法成功鉴定了20个差异蛋白质,生物信息学分析表明其中80%定位于叶绿体,主要参与光合作用和碳代谢,参与的生物过程主要是细胞过程、细胞成分组织或生物起源、生物调控、代谢过程和定位。研究表明,玫瑰散发的香气物质一方面促进烟草产生防卫反应蛋白的表达来加强其自身的抗病性,另一方面降低烟草的光合作用,抑制烤烟的生长发育。
In this experiment, two-dimensional electrophoresis and mass spectrometry were used to separate and identify K326 leaf protein between single crop and rose. Bioinformatic analysis of the identified proteins was performed using Plant-m PLoc, Gene Ontology, KOBAS, STRING. Of the 400 proteins isolated from each sample, 20 differential proteins were successfully identified by MALDI-TOF / TOF MS. Bioinformatics analysis showed that 80% of them were located in the chloroplast, mainly involved in photosynthesis and carbon metabolism. Biological processes are mainly cellular processes, cellular components or biological origin, biological regulation, metabolic processes and positioning. Studies have shown that the aroma substances released by roses promote the expression of defense-reactive protein on the one hand to enhance their own disease resistance and on the other hand reduce the photosynthesis of tobacco and inhibit the growth and development of flue-cured tobacco.