目的 :构建 HBV核心抗原 DNA疫苗 ,并探讨其在实验动物体内的表达及其免疫原性。方法 :采用 PCR法从 HBV全基因 DNA中获得核心抗原 DNA片段 ,并将其重组到 pc DNA3载体。以此为候选疫苗分别免疫小鼠和树 ,并检测其抗HBc Ig G。结果 :构建了乙肝核心抗原候选核酸疫苗 pc DNA3- HBc。免疫接种该疫苗后第 2周抗 HBc Ig G水平开始升高 ,小鼠至第 9周 ,树至第 7周升至峰值。结论 :构建的 pc DNA3- HBc DNA疫苗在小鼠及树体内获表达并诱导了体液免疫反应 Objective: To construct DNA vaccine of HBV core antigen and investigate its expression in immunized mice. Methods: The DNA fragment of core antigen was obtained from HBV whole genome DNA by PCR and recombined into pc DNA3 vector. As a vaccine candidate, mice and tree shrews were immunized and their anti-HBc Ig G was tested. Results: The hepatitis B core antigen candidate DNA vaccine pc DNA3-HBc was constructed. Anti-HBc Ig G levels began to rise in the second week after immunization of the vaccine. The mice peaked at week 9 until tree 7 in week 7. CONCLUSION: The constructed pcDNA3-HBc DNA vaccine is expressed in mice and tree shrew and induces humoral immune response