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目的:观察普罗布考对多柔比星肾病大鼠肾脏的保护作用并探讨其作用机制。方法:40只雄性SD大鼠随机分为对照组、模型组、治疗组。尾静脉一次性注射多柔比星6mg·kg~(-1)制备多柔比星肾病模型。1周后开始药物干预,模型组每天温开水灌胃,治疗组每天500mg·kg~(-1)·d~(-1)普罗布考灌胃,持续4周。4周后检测大鼠24h尿蛋白定量、血清总胆固醇(TC)、三酰甘油(TG)、尿素氮(BUN)、血清肌酐(Scr)、血清及肾组织SOD活性和MDA含量,同时取肾组织标本观察病理学改变。结果:与模型组相比,治疗组24h尿蛋白排泄量和血脂明显下降(P<0.05),肾指数明显改善(P<0.05),血清和肾组织中SOD活性明显上升、MDA含量明显下降(P<0.05),肾组织病理损伤减轻。结论:普罗布考能提高多柔比星肾病大鼠血和肾组织中SOD活性,降低血脂,减少蛋白尿,减轻肾组织的病理改变,具有肾保护作用。
Objective: To observe the protective effect of probucol on the kidneys of doxorubicin nephropathy rats and to explore its mechanism. Methods: Forty male SD rats were randomly divided into control group, model group and treatment group. A single injection of doxorubicin 6mg · kg ~ (-1) into the caudal vein for the preparation of doxorubicin nephropathy model. One week later, drug intervention was started. The model group was given warm water every day. The treatment group was given probucol 500 mg · kg -1 d -1 every day for 4 weeks. After 4 weeks, the 24h urinary protein, serum total cholesterol (TC), triglyceride (TG), blood urea nitrogen (BUN), serum creatinine (Scr), SOD activity and MDA content in serum and kidney tissue were measured. Tissue specimens observed pathological changes. Results: Compared with the model group, the urinary protein excretion and blood lipids in the treatment group decreased significantly (P <0.05) and the renal index improved significantly (P <0.05). The SOD activity in the serum and kidney tissue increased significantly and the MDA content decreased P <0.05), renal tissue pathological lesion reduced. CONCLUSION: Probucol can improve the activity of SOD in blood and kidney tissue of rats with doxorubicin nephropathy, reduce the blood lipid, reduce the proteinuria, reduce the pathological changes of kidney tissue, and have the renal protective effect.